卵巢高级别浆液性癌中p-ERK5、WT-1蛋白的表达与患者预后的关系

Expression of p-ERK5 and WT-1 protein in high-grade ovarian serous carcinoma and their relationships with prognosis of patients

目的检测卵巢高级别浆液性癌(HSOC)患者癌组织中磷酸化细胞外信号激酶5(p-ERK5)、Wilms肿瘤基因(WT-1)蛋白的表达,分析其与患者预后的关系,为HSOC患者预后判定提供依据。方法选择2008年1月至2019年12月就诊于华北理工大学附属医院妇产科的54例HSOC患者为研究对象,分别收集患者年龄、临床分期、是否淋巴转移、是否合并腹水、是否3年内复发、是否铂敏感、是否为中间型肿瘤细胞减灭术(IDS)、首诊CA125水平等资料;收集患者卵巢癌组织标本,应用免疫组织化学法检测患者卵巢癌组织中p-ERK5及WT-1蛋白表达水平,比较不同临床病理特征下两种因子的表达情况。采用Kaplan-Meier法和Cox回归分析评估HSOC患者的预后。结果p-ERK5及WT-1蛋白低表达组患者的中位无进展生存(PFS)时间分别为36.0个月和37.0个月,高表达组患者的中位PFS时间分别为17.0个月和15.0个月,p-ERK5及WT-1蛋白低表达组患者的中位总生存(OS)时间为90.0个月,高表达组患者的中位OS时间为40.0个月,组间比较差异均有统计学意义(P<0.001)。结论p-ERK5及WT-1蛋白高表达与较短的PFS时间和OS时间有关。

Objective To detect the expression of p-ERK5 and WT-1 in cancer tissues of the patients with high-grade serous ovarian cancer(HSOC),and to analyze their relationship with the prognosis of the patients to provide a basis for judging the prognosis of HSOC patients.Methods Fifty-four patients with HSOC visiting in the gynecology and obstetrics department of the Affiliated Hospital of North China University of Technology from January 2008 to December 2019 were selected as the study subjects.The age,clinical stage,lymph node metastasis,complicating ascites,recurrence within 3 years,platinum sensitive,interval debulking surgery(IDS)and CA125 level at first visit were collected.The ovarian cancer tissue samples were collected.The expression levels of p-ERK5 and WT-1 protein in ovarian cancer tissues were detected by immunohistochemistry.The expression situation of the two kinds of factors were compared among different clinicopathological features.The Kaplan-Meier method and COX regression analysis were used to evaluate the prognosis of the patients with HSOC.Results The median progress free survival(PFS)in the p-ERK5 and WT-1 protein low-expression groups was 36.0 months and 37.0 months respectively,which in the high-expression groups was 17.0 months and 15.0 months respectively.The median overall survival(OS)in the p-ERK5 and WT-1 protein low expression group was 90.0 months,which in the high expression group was 40.0 months,and the difference was statistically significant(P<0.001).Conclusion The high expression of p-ERK5 and WT-1 protein is associated with PFS time and OS time.