血清肝纤维化指标、HBV-DNA病毒载量联合检验在乙型肝炎肝纤维化中的评估价值

Evaluation of the Combined Use of Serum Liver Fibrosis Markers andHBV-DNA Viral Load in Assessing Hepatitis B-Related Liver Fibrosis

目的:探究血清肝纤维化指标、HBV-DNA病毒载量联合检验在乙型肝炎肝纤维化中的评估价值。方法:选取2022年1月至2023年5月本院收治的100例乙型肝炎肝纤维化患者作为观察组,并选择同期100例慢性乙型肝炎患者作为对照组。分别采用化学发光法和荧光定量PCR法检测两组患者肝纤维化指标及HBV-DNA水平,并比较分析其与乙型肝炎肝纤维化的关系。结果:观察组透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(Ⅳ-C)水平均显著高于对照组(P<0.05);HBV-DNA水平显著低于对照组(P<0.05)。结论:HBV在慢性乙型肝炎患者具有高度复制性,在肝纤维化阶段复制水平下降。随着乙肝的进一步发展,肝纤维化指标水平逐渐升高。联合检测肝纤维化指标和HBV-DNA病毒载量对临床诊断乙型肝炎肝纤维化有一定的参考价值。

Objective:To investigate the combined evaluation value of serum liver fibrosis markers and HBV-DNA viral load in assessing hepatitis B-related liver fibrosis.Methods:From January 2022 to May 2023,100 patients with hepatitis B-related liver fibrosis admitted to our hospital were selected as the observation group,and 100 patients with chronic hepatitis B during the same period were chosen as the control group.Serum levels of liver fibrosis markers(hyaluronic acid[HA],laminin[LN],type III procollagen[PC III],and type IV collagen[IV-C])and HBV-DNA were detected using chemiluminescence and fluorescent quantitative PCR methods,respectively.Relationships between these markers and hepatitis B-related liver fibrosis were compared and analyzed.Results:Levels of HA,LN,PC III,and IV-C were significantly higher in the observation group compared to the control group(P<0.05),while the HBV-DNA level was significantly lower in the observation group(P<0.05).Conclusion:HBV exhibits high replicative activity in patients with chronic hepatitis B,which decreases during the liver fibrosis stage.As hepatitis B progresses,levels of liver fibrosis markers gradually increase.The combined detection of liver fibrosis markers and HBV-DNA viral load provides a certain reference value for the clinical diagnosis of hepatitis B-related liver fibrosis.