酶法辅助提取桑葚多糖的体外抗氧化、抗炎活性研究

Study on Antioxidant and Anti-inflammatory Activities of Polysaccharides from Mori fructus by Enzyme-Assisted Extraction in Vitro

通过酶法辅助提取桑葚多糖,对不同浓度桑葚多糖的DPPH自由基、羟自由基、ABTS自由基和超氧阴离子自由基清除率进行测定,并建立LPS诱导Raw264.7体外炎症模型,探究桑葚多糖的抗炎活性。结果表明:当桑葚多糖浓度为1.0 mg/mL时,其对DPPH自由基、羟自由基、ABTS自由基和超氧阴离子自由基的清除率分别达到88.74%,44.96%,81.35%和56.38%。细胞活力测定结果表明,当桑葚多糖浓度为0~200μg/mL时,作用于Raw264.7细胞,未对其产生显著的毒性作用。桑葚多糖浓度为200μg/mL时,其对LPS诱导的Raw264.7细胞中NO、IL-6、IL-1β、TNF-α和COX-2抑制率分别达到33.7%,42.9%,57.1%,55.3%和56.9%。以上结果显示,酶法辅助提取的桑葚多糖具有良好的体外抗氧化和抗炎活性,旨在为天然功能性食品开发奠定基础。

The scavenging rates of DPPH,OH,ABTS+·and superoxide anion free radical of different concentrations of Mori fructus polysaccharide(MFP)were determined by enzyme-assisted extraction.The LPS-induced inflammation model of Raw264.7 in vitro was established to explore the anti-inflammatory activity of MFP.The results showed when the concentration of MFP was 1.0 mg/mL,the scavenging rates of DPPH·,OH·,ABTS^(+)·and superoxide anion free radical reached 88.74%,44.96%,81.35%and 56.38%,respectively.The results showed that there was no significant toxic effect on Raw264.7 cells when the concentration of MFP was 0-200μg/mL.When the concentration of MFP was 200μg/mL,the inhibition rates of NO,IL-6,IL-1β,TNF-αand COX-2 in Raw264.7 cells induced by LPS were 33.7%,42.9%,57.1%,55.3%and 56.9%,respectively.The above results showed that enzymatic assisted extraction of MFP had excellent antioxidant and anti-inflammatory activities in vitro,which was intended to lay a foundation for the development of natural functional food.