一种翘鳞香菇培养基配方的设计

Design of a Formula for Cultivating Scaled Mushroom Medium

翘鳞香菇是一种营养丰富且有一定药用价值的珍稀食药两用真菌,为更好种植翘鳞香菇,通过单因素试验结合正交试验法进行相关母种营养物质的设计。试验发现,翘鳞香菇最适合的碳源是果糖,氮源是酵母浸粉,无机盐是氯化钾。通过正交设计可进一步得出,当果糖16 g、酵母浸粉10 g、氯化钾1.5 g时,营养搭配最为合理,其菌丝长速为0.60 cm/d。研发的人工配方来源广泛、成本低廉,适用于翘鳞香菇的母种制作。

Lentinus squarrosulus is a kind of rare edible and medicinal fungi with rich nutrition.In order to better plant L.squarrosulus,single factor experiment and orthogonal experiment are respectively designed of its stock culture.It is found that the most suitable carbon source of this kind of mushroom is fructose and nitrogen source is yeast powder.Moreover,inorganic salt is potassium chloride.Through orthogonal design,it can be further concluded that when fructose is 16 g,yeast powder is 10 g,and potassium chloride is 1.5 g,the nutritional combination is the most reasonable,and the mycelial growth rate is 0.60 cm/d.The artificial media components have a wide range of sources and low cost,which can be applied to the preparation of stock culture of L.squarrosulus.