ELISA、CGM与ALT在出入境人员丙肝监测中的应用

Application of ELISA,CGM and ALT in Hepatitis C Survellance of Entry and Exit Personnel

本研究使用酶联免疫吸附试验(Enzyme-Linked Immunosorbent Assay,ELISA)、胶体金方法(Colloidal Gold Method,CGM)与丙氨酸氨基转氨酶(Alanine Aminotransferase,ALT)联合检测,探讨其在出入境人员丙型病毒性肝炎监测中的作用。以辽宁口岸地区出入境人员疑似丙肝的178例血液样品为研究对象,用ELISA和CGM进行丙肝抗体检测,同时检测血液样品中的ALT,统计分析检测数据。结果显示:ELISA阳性172例,灵敏度为97.26%,特异性为59.00%;CGM方法阳性139例,灵敏度为91.78%,特异性为35.24%;ELISA特异性优于CGM方法(p<0.05),丙肝病例中ALT结果异常率为61.64%,高于对照组(10.48%),有统计学意义(p<0.05);随着ELISA吸光度值升高,丙肝病例、CGM方法阳性率与ALT结果异常率呈上升趋势。结果表明,ELISA、CGM方法联合ALT检测有利于丙肝病例的及时发现,降低漏检情况发生,为更有效地做好出入境人员传染病工作提供科学依据。

In this paper,Enzyme-Linked Immunosorbent Assay(ELISA),Colloidal Gold Method(CGM)were used in combination with Alanine Aminotransferase(ALT),to explore the prevalence of hepatitis C in entry and exit personnel.At Liaoning Port Area,178 blood samples of suspected hepatitis C among entry-exit personnel were studied.Hepatitis C antibody detection in blood samples was performed by using ELISA,CGM method and ALT,and the detection data was statistically analyzed.According to the results,172 cases were ELISA positive,with a sensitivity of 97.26%and a specificity of 59.00%;139 cases were CGM method positive,with a sensitivity of 91.78%and a specificity of 35.24%.The specificity of ELISA was better than CGM method(p<0.05).The abnormal result rate of ALT in Hepatitis C cases was 61.64%,higher than the control group(10.48%),with statistical significance achieved(p<0.05);As the ELISA absorbance value increased,the positive rates of Hepatitis C cases,CGM method positive cases,and ALT abnormal results showed an upward trend.The results showed that the combination of ELISA、CGM methods and ALT detection is beneficial for timely detection of Hepatitis C cases and reduces the occurrence of missed detections,thus providing scientific basis for more effective work on infectious diseases of entry and exit personnel.