分子印迹-Ru(bpy)_(3)^(2+)探针电化学传感器法测定食品中的组胺

Electrochemical Sensor for the Determination of Histamine in Food by Molecular Imprinting-Ru(bpy)_(3)^(2+)Probe System

组胺是人体内一种重要的生物化学物质,但摄入过量会导致组胺不耐症和组胺中毒等问题。因此,开发一种高选择性、低成本的检测食品中组胺的方法尤为重要。本研究以组胺为模板分子,邻苯二胺为功能单体,利用电化学聚合的方法,在玻碳电极表面制备了组胺分子印迹传感器。传感器利用三联吡啶钌(Ru(bpy)_(3)^(2+))作为探针,借助循环伏安特性曲线,以电极对组胺分子在传感器上的吸附、洗脱后电流和组胺浓度的定量关系为工作原理,测定了几种食品基质中的组胺含量。同时,对该分子印迹电流传感器的选择性、重现性以及稳定性进行了考察。传感器的检测线性浓度范围为1.0×10^(-5)~1.0×10^(-7)mol/L,相关系数为0.993,检出限为8.4×10^(-8)mol/L(3σ/S),加标回收率在91.2%~106.6%之间,RSD值小于5%。该方法操作简单,具有很好的特异性,可适用于食品中组胺的检测。

Histamine(HA)is an important biochemical substance in the human body,but excessive consumption of HA can lead to problems such as histamine intolerance and histamine poisoning.Therefore,it is particularly necessary to develop selective and lowcost detection methods for HA in food.In this study,an HA molecular imprinting sensor was prepared on the surface of a glassy carbon electrode using HA as a template molecule and o-phenylenediamine as a functional monomer through electrochemical polymerization.The sensor used tris(2-pyridyl)ruthenium(Ru(bpy)_(3)^(2+))as a probe,and based on the quantitative relationship between histamine concentration and current after adsorption and elution of histamine molecules on the sensor,the content of histamine in several food substrates was determined by cyclic voltammetry characteristic curve.The detection linear concentration range of the prepared sensor was 1.0×10^(-5)to 1.0×10^(-7)mol/L,with a correlation coeficient of 0.993 and a detection limit of 8.4×10^(-8)mol/L(3 o/S).The recovery rate of spiked samples was between 91.2%and 106.6%,with an RSD value of less than 5%.This method is simple to operate,has good specificity,and can be applied to the detection of HA in different food substrates.