ADSCs对大鼠子宫内膜机械损伤后修复、胶原蛋白水平及PPAR-α/FAO信号的影响

Effects of ADSCs on endometrial repair,collagen level and PPAR-α/FAO signal after mechanical injury in rats

目的探讨脂肪干细胞(ADSCs)对大鼠子宫内膜机械损伤后修复、胶原蛋白水平及PPAR-α/FAO信号的影响。方法23只大鼠建立子宫内膜机械损伤模型,成功21只并随机分为模型组、ADSCs组、雌激素组3组,每组7只,未建立模型的7只为正常组。ADSCs组取ADSCs细胞悬液进行尾静脉注射,雌激素组对大鼠进行皮下注射17β-雌二醇;其余组大鼠不做干预。观察各组大鼠子宫内膜病理组织形态、子宫内膜厚度及腺体数量、子宫内膜组织VEGF及FGF水平、Ⅰ型胶原蛋白水平及PPAR-α/FAO信号通路蛋白质表达。结果正常组大鼠子宫内膜形状正常,模型组大鼠子宫内膜最差,而雌激素组及ADSCs组大鼠子宫内膜形态改善均优于模型组;各组间子宫内膜厚度及腺体数量比较,雌激素组、ADSCs组优于模型组,且ADSCs组升高明显(P<0.05);各组间血清VEGF、FGF表达水平比较,雌激素组、ADSCs组优于模型组,且ADSCs组升高明显(P<0.05);各组间Ⅰ型胶原蛋白、PPAR-α、FAO表达比较,雌激素组、ADSCs组优于模型组,且ADSCs组升高明显(P<0.05)。结论ADSCs可改善子宫内膜形态组织,增加子宫内膜厚度、腺体数量及Ⅰ型胶原蛋白,促进子宫内膜分泌VEGF及FGF因子,调控PPAR-α/FAO信号通路蛋白质表达,对子宫内膜机械损伤进行修复。

Objective To investigate the effects of ADSCs on endometrial repair,collagen level and PPAR-α/FAO signal after mechanical injury in rats.Methods The mechanical injury model of endometrium was established in 23 rats,and 21 rats were successfully divided into model group,ADSCs group and estrogen group randomly,with 7 rats in each group,and 7 rats without model were normal group.ADSCs cell suspension was injected into tail vein in ADSCs group,and 17β-estradiol was injected subcutaneously in estrogen group.The other groups of rats did not intervene.The pathological tissue morphology,endometrial thickness,number of glands,levels of VEGF and FGF,type I collagen and PPAR-α/FAO signaling pathway protein were observed in each group.Results The endometrial shape of rats in normal group was normal,the endometrial shape of rats in model group was the worst,and the endometrial shape of rats in estrogen group and ADSCs group was better than that in model group.The endometrial thickness and number of glands in estrogen group and ADSCs group were better than model group,and ADSCs group was significantly increased(P<0.05).The expression levels of serum VEGF and FGF in estrogen group and ADSCs group were higher than those in model group,and ADSCs group were significantly increased(P<0.05).The expression of type I collagen,PPAR-αand FAO among all groups was higher in estrogen group and ADSCs group than in model group,and the ADSCs group was significantly increased(P<0.05).Conclusion ADSCs can improve endometrial morphological tissue,increase endometrial thickness,number of glands and type I collagen,promote endometrial secretion of VEGF and FGF factors,regulate PPAR-α/FAO signaling pathway protein expression,and repair the mechanical injury of endometrial.