一测多评法测定甘草及其不同炮制品中7种成分含量

Determination of seven components in licorice and its different processed products by QAMS

为了实现对甘草药材及其炮制品成分的多指标质量评价,建立了一测多评测定甘草药材及3种炮制品中7种化学成分含量的方法。以甘草苷为内标,计算异甘草苷相对校正因子;以甘草酸为内标,计算芹糖甘草苷、芹糖异甘草苷、新异甘草苷、甘草素的相对校正因子,再测定各成分含量,并将计算结果与外标法测定结果进行比较。结果表明:一测多评法测定甘草及其不同炮制品中7种成分的含量具有可行性;甘草炮制后,其中的甘草苷、甘草酸、芹糖甘草苷、芹糖异甘草苷、新异甘草苷的含量均有所降低,且在不同炮制品中的含量呈现出相同的变化趋势,均为ω(甘草药材)>ω(炒甘草)>ω(甘草片)>ω(炙甘草)。所建立的一测多评法稳定、准确,且重复性好,可为甘草及其不同炮制品的质量评价提供参考。

In order to achieve the quality evaluation of multiple indicator components in licorice and its processed products,a quantitative analysis of multi-components by single marker(QAMS)was established to determine the content of seven chemical components in licorice medicinal materials and three different processed products.The calibration factor for isoliquiritigenin was calculated by using glycyrrhizin as the internal standard;The relative correction factors of apigenin glycyrrhizic acid,apigenin isoliquiritigenin,neoisoliquiritigenin,and glycyrrhizin were calculated by using glycyrrhetinic acid as the internal standard,and then the content of each component was determined.The calculated results were compared with the results obtained by the external standard method.The results show that the QAMS is feasible for determining the content of seven components in licorice and its different processed products.After processing,the content of glycyrrhizin,glycyrrhetinic acid,apigenin glycyrrhizin,apigenitigenin,and neoisoglycyrrhizin all decreases and shows the same trend of change.The trend of content change isω(licorice herbs)>ω(fried licorice)>ω(licorice tablets)>ω(roasted licorice).The established method is stable,accurate,and has good repeatability,which can provide reference for the quality evaluation of licorice and its different processed products.