南方红豆杉全长转录组测序及生物信息学分析

Full-length Transcriptome Sequencing and Bioinformatics Analysis of Taxus chinensis var.mairei

为了充分挖掘南方红豆杉功能基因,开发利用其生物资源。选用PacBio Sequel第三代测序技术,对贵州省梵净山地区的南方红豆杉植株进行转录组测序,开展生物信息学分析。共获得68.03 Gb原始数据,包含1001302条原始序列读取片段。经组装、拼接、去冗余后获得65152条单基因簇(Unigene),平均长度为2591 bp。与已有数据库比对显示,南方红豆杉参与细胞和代谢活动的基因表达量较高,具有较强的代谢活动和遗传信息处理能力,转录组中与生长发育、抗逆、次生代谢和合成相关的转录因子,为全面开发南方红豆杉和遗传育种提供数据支持。

In order to fully explore the functional genes and utilize the biological resources of Taxus chinensis var.mairei,PacBio Sequel third-generation sequencing technology was used to conduct high-throughput transcriptome sequencing,and systematic bioinformatics analysis was employed to study the Taxus chinensis var.mairei obtained from Mount Fanjing,Guizhou Province.The study obtained 68.03 Gb of raw data,which included 1001302 raw sequence reading fragments.After assembling,splicing and de-redundancy,65152 unigenes with an average length of 2591 bp were obtained.Database comparison showed that the gene expression of cellular and metabolic activities of the Taxus chinensis var.mairei were higher,indicating strong metabolic activity and genetic information processing capacity.Meanwhile,transcription factors in the transcriptome related to growth and development,stress resistance,secondary metabolism and synthesis provided new insight into the study of the comprehensive utilization and genetic breeding of Taxus chinensis var.mairei.