硫自养反硝化ASBR工艺脱氮除硫效果及功能菌研究

Simultaneous nitrogen and sulphur removal effect and functional bacteria of sulfur-driven autotrophic denitrification ASBR process

研究了硫自养反硝化-厌氧序批式反应器(SDAD-ASBR)同步脱氮除硫效果的影响因素和SDADASBR中微生物群落结构特征。结果表明,当n(S)/n(N)(记作S/N)为1,进水S^(2-)和NO_(3)^(-)-N质量浓度分别为300 mg/L和131.25 mg/L时系统内无亚硝酸盐积累现象,脱氮除硫效率高,硫化物和硝酸盐的去除率分别为99.97%和84.34%;但S/N降低,出水产物中亚硝酸盐升高。水力停留时间(HRT)对硝酸盐的去除效果影响显著,但对硫化物的去除效果影响不大,当HRT由12 h缩短至2.4 h时,硫化物的去除率均在99%以上,而硝酸盐的去除率则由93.14%下降至77.04%。系统中脱氮除硫的主要功能菌属为微小杆菌属、梭菌属、芽孢杆菌属、硫杆菌属和鞘单胞菌属。

In this paper,the influence factors of nitrogen and sulphur removal effect and main functional bacteria were investigated in a sulfide autotrophic denitrification-anaerobic sequencing batch reactor(SDAD-ASBR).The re-sults showed that when n(S)/n(N)(recorded as S/N)was 1,and the influent S^(2-) and NO_(3)^(-) -N were 300 mg/L and 131.25 mg/L,respectively,there was no nitrite formation,and the sulfides and nitrates removal rates were 99.97%and 84.34%,respectively.However,as the S/N was reduced,the concentration of nitrite in the effluent increased.The hydraulic retention time(HRT)had a significant effect on the removal of nitrate,but had little effect on the sul-phide removal.When HRT was shortened from 12 h to 2.4 h,the removal rates of sulfide were all above 99%,while the removal rate of nitrate decreased from 93.14%to 77.04%.The main functional genera for nitrogen and sulphur removal in the system were Exiguobacterium,Clostridium,Bacillus,Thiobacillus and Sphingomonas.