乳腺浸润性导管癌超声特征与促血管生成相关基因的关系研究

Relationship Between Ultrasonographic Characteristics and Angiogenesis Related Genes in Breast Invasive Ductal Carcinoma

目的探讨乳腺浸润性导管癌超声特征与促血管生成相关基因之间的关系。方法利用PubMed数据库检索并筛选促乳腺癌血管生成相关基因。使用STRING数据库及Cytoscape软件构建蛋白互作网络(protein-protein interaction networks,PPI),并筛选关键基因。收集2022年1月~2023年8月经病理证实为乳腺浸润性导管癌的女性患者38例。分析患者临床及超声特征与关键基因之间的关系。结果共筛选出10个关键基因。年龄>50岁组患者的TGF-β1及CXCL12表达量大于年龄≤50岁组(P<0.05)。有淋巴结转移组患者的白细胞介素-6(interleukin-6,IL-6)表达量大于无淋巴结转移组(P<0.05)。超微血流成像技术(ultra micro angiography,UMA)的血流像素比(color pixel percentage,CPP)>10%组患者的CCL2表达量大于UMA的CPP≤10%组(P<0.05)。血管主要分布在周边组患者的Akt1表达量大于血管主要分布在中心组(P<0.05)。造影灌注不均匀组患者的IL-6、TGF-β1、PECAM1、CXCL8及CXCL12表达量大于造影灌注均匀组(P<0.05)。造影灌注缺损面积>1/2组患者的TGF-β1、PECAM1、FN1、CXCL12表达量大于造影灌注缺损面积≤1/2组(P<0.05)。参与调节造影灌注均匀性和灌注缺损面积的基因存在相关性,特别是两者的交集基因TGF-β1、PECAM1、CXCL12(P<0.05)。结论乳腺浸润性导管癌超声特征与促血管生成关键基因之间具有相关性,可以为乳腺癌个体化治疗提供参考依据。

Objective To explore the relationship between ultrasonographic characteristics and promoting angiogenesis related genes in breast invasive ductal carcinoma.Methods A search was conducted in the PubMed database to identify genes related to promoting angiogenesis in breast cancer.Protein-protein interaction networks(PPI)were constructed using the STRING database and Cytoscape software,and screen for key genes.A total of 38 female patients diagnosed with breast invasive ductal carcinoma from January 2022 to August 2023 were included in the study.The relationship between clinical and ultrasonographic characteristics of these patients and key genes was analyzed.Results A total of 10 key genes were screened.The expression levels of TGF-β1 and CXCL12 in patients aged>50 years were higher than those in patients aged≤50 years(P<0.05).The expression level of IL-6 in patients with lymph node metastasis was higher than that in patients without lymph node metastasis(P<0.05).The expression level of CCL2 in patients with color pixel percentage(CPP)of ultra micro angiography(UMA)>10%was higher than that in patients with CPP of UMA≤10%(P<0.05).The expression level of Akt1 in patients with predominantly peripheral vascular distribution were higher than that in patients with central distribution(P<0.05).The expression levels of IL-6,TGF-β1,PECAM1,CXCL8 and CXCL12 in patients with uneven contrast perfusion were higher than those in patients with even contrast perfusion(P<0.05).The expression levels of TGF-β1,PECAM1,FN1 and CXCL12 in patients with contrast perfusion defect area>1/2 were higher than those in patients with contrast perfusion defect area≤1/2(P<0.05).There was a correlation among genes involved in regulating contrast perfusion uniformity and perfusion defect area,particularly the intersecting genes TGF-β1,PECAM1 and CXCL12(P<0.05).Conclusion There is a correlation between the ultrasonographic characteristics of breast invasive ductal carcinoma and key genes related to promoting angiogenesis,which may provide a reference for individualized treatment of breast cancer.