造血干细胞标志分子CD34的结构与功能新认识

Novel insights on the structure and function of hematopoietic stem cell marker CD34

CD34是造血干/祖细胞最主要的膜表面标志物,是临床上造血干细胞移植(hematopoietic stem cell transplanta-tion,HSCT)中评估和选择供体移植物的核心指标。然而,令人惊讶的是,CD34的生物学功能至今仍然知之甚少。尽管已有研究揭示了CD34在介导黏附中发挥重要作用,但其发挥促进还是抑制功能尚无定论。CD34作为一种高度糖基化的Ⅰ型跨膜蛋白,糖基化、磷酸化等翻译后修饰可能对于其构象及功能具有重要调控作用。本实验室前期工作发现了CD34共价活性位点、磷酸化修饰位点及其潜在功能。本综述结合基础研究实验结果,对CD34分子研究进展作一简要介绍和展望。

CD34 is the major membrane surface marker of hematopoietic stem/progenitor cells,and the core index for evaluating and selecting donor grafts in clinical hematopoietic stem cell transplantation(HSCT).However,the biological function of CD34 remains poorly understood.Functional studies of CD34 have revealed its important role in mediating adhesion,whether it functions as a facilitator or an inhibitor has not yet been determined.As a highly glycosylated type I transmembrane protein,the post-translational modifications such as glycosylation and phosphorylation may play an important role in the regulation of CD34 conformation and function.Our previous research has identified the covalent active sites and phosphorylation sites of CD34 and their potential functions.In this review,a brief introduction and prospection on the molecular research progress of CD34 through combining basic research results was presented.