基于UPLC-QTOF/MS的非靶向代谢组学构建2型糖尿病患者血浆代谢轮廓的研究

Construction of plasma metabolic profile of type 2 diabetic mellitus patients based on UPLC-QTOF/MS non-targeted metabonomics.

目的探究维吾尔族和哈萨克族2型糖尿病(T2DM)患者及正常糖耐量(NGT)人血浆代谢物的变化,构建差异代谢物的轮廓及分析不同代谢途径。方法收集维吾尔族和哈萨克族T2DM及NGT的血浆样本,用超高效液相色谱串联四极杆飞行时间质谱进行检测。用MetaboAnalyst 5.0进行筛选,条件为P<0.05和差异倍数(FC)<0.90或FC>1.10,并用京都基因和基因组百科全书数据库富集分析差异代谢物的代谢通路。结果维吾尔族T2DM组与NGT组相比,有109种的差异代谢物,特有的43种差异代谢物主要富集在苯丙氨酸、酪氨酸和色氨酸的生物合成等5个通路中。哈萨克族的T2DM与NGT组相比,有86种差异代谢物,特有的28种差异代谢物主要富集在尿素循环、精氨酸和脯氨酸代谢(impact>0.10,P<0.05)。结论本文构建了T2DM人血浆代谢轮廓谱,并筛选出维吾尔族和哈萨克族T2DM特有的代谢物,为T2DM早期预防提供潜在生物标志物。

Objective To explore the changes of plasma metabolites in Uygur and Kazak patients with type 2 diabetes mellitus(T2DM)and people with normal glucose tolerance(NGT),construct the profile of different metabolites and analyze different metabolic pathways.Methods Plasma samples of T2 DM and NGT from Uygur and Kazak were collected for detection.Ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry was used for detection.MetaboAnalyst 5.0 was used to analyze the data,and the screening conditions were P<0.05 and fold change(FC)<0.90 or FC>1.10.We used The Kyoto Encyclopedia of Genes and Genomes database to enrich and explore the metabolic pathways of different metabolites.Results Compared with the Uighur NGT group,there are109 differential metabolites in the T2DM group,and 43 unique differential metabolites are mainly concentrated in five pathways,such as phenylalanine biosynthesis,tyrosine biosynthesis,and tryptophan metabolism.Compared with the NGT group,Kazak T2DM has 86differential metabolites,and 28 unique differential metabolites are mainly enriched in the urea cycle,arginine,and proline metabolism(impact>0.10,P<0.05).Conclusion In this study,the plasma metabolic profile of T2DM patients was constructed,and the unique metabolites of T2DM in Uygur and Kazak were screened out,which provided potential biomarkers for early prevention of T2DM.