富氢水对急性肝损伤小鼠模型的保护作用研究

Study on the Protective Effect of Hydrogen-rich Water on Acute Liver Injury Model in Mice

目的 研究富氢水对小鼠急性肝损伤的保护作用。方法 60只雄性KM小鼠随机分为空白组、模型组、阳性对照组、富氢水低剂量组、富氢水中剂量组和富氢水高剂量组。除空白组外,各组小鼠腹腔注射0.3%的CCl4制备急性肝损伤模型。观察肝脏的形态变化,计算肝指数,HE染色观察肝脏病理变化并进行病理评分。检测小鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、总胆红素(TBIL-F)、直接胆红素(DBIL-F)的浓度。此外,分别收集空白组、模型组和富氢水中剂量处理组小鼠的肝脏组织(n=5)进行转录组测序。结果 与空白组相比,模型组肝组织颜色变浅,表面出现纹理,病理评分增高。与模型组相比,富氢水组肝组织损伤评分降低,肝指数降低,血清中ALT、AST、TBIL-F、DBIL-F浓度降低,富氢水组有100个基因上调,194个基因下调,共计294个差异基因。结论 富氢水可通降低血清中ALT、AST、TBIL-F、DBIL-F的浓度,使肝脏组织中的基因表达特征向空白组靠近,降低肝损伤程度,最终在急性肝损伤小鼠模型中起到保肝作用。

Objective To study the protective effect of hydrogen-rich water on acute liver injury in mice.Methods Sixty male KM mice were randomly divided into blank group,model group,positive control group,hydrogen-rich water low dose group,hydrogen-rich water dose group and hydrogen-rich water high dose group.Except for the blank group,mice in each group were intraperitoneally injected with 0.3%CCl4 to prepare acute liver injury model.The morphological changes of the liver were observed,the liver index was calculated,and the pathological changes of the liver were observed and scored by HE staining.The concentrations of alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBIL-F)and direct bilirubin(DBIL-F)in serum of mice were detected.In addition,the liver tissues(n=5)of mice in the blank control group,model group and hydrogen-rich water dose treatment group were collected for transcriptome sequencing.Results Compared with the blank group,the color of liver tissue in the model group became lighter,the surface appeared texture,and the pathological score increased.Compared with the model group,the liver tissue injury score of the hydrogen-rich water group decreased,the liver index decreased,and the concentrations of ALT,AST,TBIL-F,and DBIL-F in the serum decreased.In the hydrogen-rich water group,100 genes were up-regulated and 194 genes were down-regulated,a total of 294 differentially expressed genes.Conclusion Hydrogen-rich water can reduce the concentration of ALT,AST,TBIL-F and DBIL-F in serum,make the gene expression characteristics in liver tissue close to the blank control group,reduce the degree of liver injury,and finally play a protective role in the mouse model of acute liver injury.