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酶联免疫吸附法与RT-PCR法对血液标本内乙肝病毒检验的价值分析

Value of Enzyme-linked Immunosorbent Assay and RT-PCR in the Detection of Hepatitis B Virus in Blood Samples
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摘要 目的 分析酶联免疫吸附法与RT-PCR法对血液标本内乙肝病毒检验的价值。方法 选取2020年3月-2023年3月我院诊治的63例疑似乙型肝炎患者为研究对象,均采用酶联免疫吸附法(ELISA)、荧光定量PCR法(RT-PCR)检测,观察两种检验方法窗口期、诊断效能(灵敏度、特异度、准确率、阳性预测值、阴性预测值)、PCR法检测(大三阳、小三阳、急性或慢性感染期)HBV-DNA结果、HBV-DNA与血清标志物(HBsAg、HBeAg)阳性率。结果 RT-PCR法窗口期为(21.12±4.54)d,短于ELISA法的(36.20±5.84)d(P<0.05);RT-PCR法灵敏度、特异度、准确率、阳性预测值、阴性预测值均高于ELISA法(P<0.05);大三阳患者HBV-DNA阳性率、HBV-DNA定量均高于小三阳、急性或慢性感染期患者,且小三阳患者高于急性或慢性感染期患者(P<0.05);BV-DNA阳性患者多为HBsAg携带者,但HBsAg、HBeAg检出符合率比较,差异无统计学意义(P>0.05)。结论 ELISA法与RT-PCR法对血液标本内乙肝病毒检验均具有一定的价值,RT-PCR法窗口期短,可提高诊断效能,且可对HBV-M进行定量检测。但ELISA法则能够对HBV-M进行定性检测,二者具有互为作用,临床可联合应用,以为乙型肝炎的治疗提高可靠的参考。 Objective To analyze the value of enzyme-linked immunosorbent assay and RT-PCR in the detection of hepatitis B virus in blood samples.Methods A total of 63 patients with suspected hepatitis B diagnosed and treated in our hospital from March 2020 to March 2023 were selected as the research objects.All patients were detected by enzyme-linked immunosorbent assay(ELISA)and fluorescence quantitative PCR(RT-PCR).The window period,diagnostic efficacy(sensitivity,specificity,accuracy,positive predictive value,negative predictive value),HBV-DNA results detected by PCR(large three positive,small three positive,acute or chronic infection period),HBV-DNA and serum markers(HBsAg,HBeAg)positive rate were observed.Results The window period of RT-PCR was(21.12±4.54)d,which was shorter than(36.20±5.84)d of ELISA(P<0.05).The sensitivity,specificity,accuracy,positive predictive value and negative predictive value of RT-PCR were higher than those of ELISA(P<0.05).The positive rate of HBV-DNA and HBV-DNA quantification in patients with large three positive were higher than those in patients with small three positive,acute or chronic infection,and patients with small three positive were higher than those in patients with acute or chronic infection(P<0.05).BV-DNA positive patients were mostly HBsAg carriers,but there was no significant difference in the coincidence rate of HBsAg and HBeAg detection(P>0.05).Conclusion ELISA method and RT-PCR method have certain value in the detection of hepatitis B virus in blood samples.RT-PCR method has a small window period,which can improve the diagnostic efficiency and quantitatively detect HBV-M.However,the ELISA method can be used to qualitatively detect HBV-M.The two have mutual effects and can be combined in clinical practice to improve the reliable reference for the treatment of hepatitis B.
作者 吴小玲 WU Xiaoling(Laboratory Department of Yugan County Maternal and Child Health Hospital,Yugan 335100,Jiangxi,China)
出处 《医学信息》 2024年第19期152-155,共4页 Journal of Medical Information
关键词 酶联免疫吸附法 RT-PCR法 血液标本 乙肝病毒 Enzyme-linked immunosorbent assay method RT-PCR method Blood samples Hepatitis B virus
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