MCM-48纳米颗粒作为小核酸载体的性能研究

Research on the Performance of MCM-48 Nanoparticles as Delivery Vectors for Small Nucleic Acid

合成制备了MCM-48型的介孔纳米颗粒,对其进行了系统的物化表征,表明其是具有高比表面积、高度有序结构和三维特征孔道特点的纳米颗粒。对MCM-48纳米颗粒进行氨基化修饰,得到N-MCM-48纳米颗粒,研究了其作为小核酸载体的性能。结果显示:N-MCM-48纳米颗粒具有良好的吸附和释放小核酸的能力、细胞摄入能力和生物相容性,并能够负载siRNA进入HepG2细胞并诱导肿瘤细胞凋亡。

MCM-48 silica nanoparticles were prepared and characterized,which showed a highly ordered mesoporous structure with Ia3d crystal lattice.The MCM-48 nanoparticles were modified with NH2-group,as N-MCM-48 nanoparticles.N-MCM-48 nanoparticles were researched as delivery vectors for small nucleic acid.The results indicated that the N-MCM-48 nanoparticles had good adsorption and release of small nucleic acid capabilities,cell uptake ability,and biocompatibility,and could load siRNA into HepG2 cells and induce tumor cell apoptosis.