肺腺癌患者胸腔积液沉渣包埋组织中甲状腺转录因子-1、半乳糖苷酶类-3表达水平及其临床意义

The expression levels of thyroid transcription factor-1 and galectin-3 in pleural fluid sediment embedded tissues of lung adenocarcinoma patients and their clinical significance

目的探讨肺腺癌患者胸腔积液沉渣包埋组织中甲状腺转录因子-1(TTF-1)、半乳糖苷酶类-3(Galectin-3)表达水平及其临床意义。方法回顾性选择2020年3月至2022年12月在安庆市第一人民医院接受诊治的82例肺癌患者为研究对象,按照肺腺癌类型分为转移性肺腺癌组(16例)和原发性肺腺癌组(66例)。采用免疫组织化学法检测两组胸腔积液沉渣包埋组织中TTF-1、Galectin-3表达水平并进行比较。分析TTF-1、Galectin-3表达水平与肺腺癌临床病理特征的关系。结果原发性肺腺癌组TTF-1阳性率高于转移性肺腺癌组[77.27%(51/66)比2/16],差异有统计学意义(χ^(2)=23.64,P<0.01)。原发性肺腺癌组Galectin-3阳性率低于转移性肺腺癌组[31.82%(21/66)比13/16],差异有统计学意义(χ^(2)=12.96,P<0.01)。Galectin-3和TTF-1阳性表达与肺腺癌的组织分化程度、淋巴结转移及临床分期无相关性(P>0.05)。结论肺腺癌患者胸腔积液沉渣包埋组织中TTF-1表达水平升高,Galectin-3表达水平降低,TTF-1、Galectin-3联合检测对诊断原发性肺腺癌细胞及鉴别诊断转移性癌具有临床应用价值。

Objective To study the expression levels and clinical significance of thyroid transcription factor-1(TTF-1)and galectin-3 in pleural fluid sediment embedded tissues of lung adenocarcinoma patients.Methods A study was conducted on the clinical data of 82 lung cancer patients who received treatment at the First People′s Hospital of Anqing City from March 2020 to December 2022.According to the type of lung adenocarcinoma,they were divided into metastatic lung adenocarcinoma group(16 cases)and primary lung adenocarcinoma group(66 cases).The expression of levels TTF-1 and galectin-3 were compared between the two groups.Immunohistochemical method was used to detect the expression levels of TTF-1 and galectin-3 in pleural fluid sediment embedded tissues and their clinical significance was analyzed.Results The positive rate of TTF-1 in primary lung adenocarcinoma group was higher than that in metastatic lung adenocarcinoma group:77.27%(51/66)vs.2/16,there was statistical difference(χ^(2)=23.64,P<0.01).The positive rate of galectin-3 in primary adenocarcinoma group was lower than that in metastatic adenocarcinoma group:31.82%(21/66)vs.13/16,there was statistical difference(χ^(2)=12.96,P<0.01).Galectin-3 and TTF-1 positive expressions were not correlated with the degree of tissue differentiation,lymph node metastasis and clinical stage of lung adenocarcinoma(P>0.05).Conclusions The expression level of TTF-1 increases and the expression level of galectin-3 decreases in the pleural fluid sediment embedded tissues of lung adenocarcinoma patients,the combined detection of TTF-1 and galectin-3 has important clinical application value in the diagnosis of primary lung adenocarcinoma cells and differential diagnosis of metastatic cancer.