卵巢癌组织长链非编码RNA PVT1表达及其靶基因生物信息学预测研究

Expression of long non-coding RNA PVT1 and bioinformatics prediction of its target gene in ovarian cancer

目的检测长链非编码RNA浆细胞瘤多样异位基因1(lncRNA PVT1)在卵巢癌组织中的表达并预测其靶基因,进而对靶基因进行生物信息学分析。方法收集2019年6月至2019年12月于我院行手术治疗的125例卵巢癌患者的癌组织及其切缘正常组织。RT-PCR检测癌组织及其切缘正常组织lncRNA PVT1的表达;比较不同临床病理特征患者卵巢癌组织lncRNA PVT1表达;应用在线工具Annolnc2数据库、GEPIA2数据库、UALCAN数据库分别筛选出lncRNA PVT1靶基因取交集;采用DAVID数据库对筛选出的lncRNA PVT1靶基因进行GO功能和KEGG通路富集分析。结果与切缘正常组织相比,癌组织中lncRNA PVT1相对表达水平升高(P<0.05);TNM分期Ⅲ~Ⅳ期、低/未分化程度的卵巢癌患者癌组织中lncRNA PVT1的相对表达水平分别高于TNM分期Ⅰ~Ⅱ期、中/高分化程度的卵巢癌患者(P<0.05);lncRNA PVT1的靶基因有44个,GO功能分析发现靶基因功能主要富集于细胞周期调控、RNA聚合酶Ⅱ启动子的转录调控、转录因子活性、赖氨酰氧化酶活性、细胞外基质、细胞质等;KEGG通路分析显示其参与的通路主要富集于癌症相关信号通路、转化生长因子-β(TGF-β)信号通路、细胞外基质受体的相互作用、环磷酸鸟苷酸(cGMP)-蛋白激酶G(PKG)信号通路、磷脂酰肌醇3激酶(PI3K)-蛋白激酶B(AKT)信号通路等。结论lncRNA PVT1在卵巢癌组织中表达上调,其靶基因富集于细胞组分、分子功能和生物调节等多个生物学过程及TGF-β、cGMP-PKG等肿瘤相关信号通路上,lncRNA PVT1通过调控靶基因的表达在卵巢癌中发挥作用。

Objective To detect the expression of long non-coding RNA plasma-cytoma variant translocation gene 1(lncRNA PVT1)in ovarian cancer tissues and predict its target gene,and then conduct bioinformatics analysis of the target gene.Methods The cancer tissues and incisal margin normal tissues of 125 patients with ovarian cancer who underwent surgical treatment in our hospital from June 2019 to December 2019 were collected.The expression of lncRNA PVT1 in cancer tissues and incisal margin normal tissues was detected by RT-PCR.The expression of lncRNA PVT1 in ovarian cancer tissues of patients with different clinicopathological characteristics was compared.The lncRNA PVT1 target genes were screened out by the online tools Anolnc2 database,GEPIA2 database,and UALCAN database to obtain the intersection.DAVID database was used to analyze the GO function and KEGG pathway enrichment of the screened lncRNA PVT1 target gene.Results Compared with incisal margin normal tissues,the relative expression of lncRNA PVT1 in cancer tissues significantly increased(P<0.05).The relative expression of lncRNA PVT1 in cancer tissues of ovarian patients with TNM stageⅢtoⅣ,poorly differentiated or undifferentiation were higher than those of ovarian patients with TNM stageⅠtoⅡ,moderately or well differentiated,respectively(P<0.05).There were 44 target genes of lncRNA PVT1.GO function analysis found that the target gene functions were mainly enriched in cell cycle regulation,transcription regulation of RNA polymeraseⅡpromoter,transcription factor activity,lysine oxidase activity,extracellular matrix,cytoplasm,etc.KEGG pathway analysis showed that the pathway it participated in were mainly enriched in cancerrelated signal pathway,transforming growth factor-β(TGF-β)signal pathway,extracellular matrix receptor interaction,cyclic guanosine phosphate(cGMP)-protein kinase G(PKG)signal pathway,phosphatidylinositol 3-kinases(PI3K)-RAC-alpha serine/threonine-protein kinase(AKT)signal pathway,etc.Conclusion The expression of lncRNA PVT1 is up-regulated in ovarian cancer tissues.Its target genes are enriched in multiple biological processes such as cell components,molecular functions,biological regulation and tumor-related signaling pathways such as TGF-β,CGMP-PKG.lncRNA PVT1 plays a role in ovarian cancer by regulating the expression of target genes.