基于干燥工艺对比两种寄主肉苁蓉中苯乙醇苷类成分含量

Comparing contents of phenylethanol glycosides in two different host Cistanches Herba after different drying treatments

利用超高效液相色谱-三重四极杆串联质谱联用法(ultra-high performance liquid chromatography-triple quadrupole mass spectrometry, UPLC-QQQ-MS)比较2种寄主肉苁蓉经过不同干燥处理后的10种苯乙醇苷类成分含量。将肉苁蓉干燥品粉碎,粉末过60目筛,用50%(体积分数)甲醇溶解后超声提取,用Agilent Eclipse Plus C18柱(2.1 mm×150 mm, 1.8μm)分离,以A相(0.1%甲酸水溶液)-B(乙腈)相作为流动相进行梯度洗脱,利用电喷雾离子源,在UPLC-QQQ-MS的动态多反应模式下检测。结果表明,不同干燥处理的寄主肉苁蓉苯乙醇苷类成分含量具有明显差异,冷冻干燥处理所得的肉苁蓉中10种苯乙醇苷类含量最高,其次为80℃烘干样品。虽然冷冻干燥对于肉苁蓉所保留10种苯乙醇苷类成分最高,但基于大规模生产考虑,80℃烘干更具有经济效益。

This study aimed to compare the content of 10 phenylethanoid glycosides in different processed Cistanches Herba from different hosts using ultra-high performance liquid chromatography-triple quadrupole mass spectrometry(UPLC-QQQ-MS).The dried Cistanches Herba was crushed,sieved through a 60-mesh sieve,and extracted by ultrasound with 50%methanol.Separation was performed on an Agilent Eclipse Plus C18 column(2.1 mm×150 mm,1.8μm)using gradient elution with mobile phase A(0.1%formic acid aqueous solution)and B(acetonitrile).Detection was carried out in dynamic multiple reaction monitoring mode using an electrospray ionization source in UPLC-QQQ-MS.Results showed that significant variations were observed in the composition of 10 phenylethanoid glycosides among Cistanches Herba cultivated on different hosts and subjected to various drying treatments.Freeze-dried Cistanches Herba showed the highest content of these compounds for both host plants,followed by drying at 80℃.Although freeze-drying preserved the maximum content of 10 phenylethanoid glycosides in Cistanches Herba,drying at 80℃was more cost-effective for large-scale production.