草地贪夜蛾核型多角体病毒Hub1的稳定性及对草地贪夜蛾的田间防治效果

Stability of Spodoptera frugiperda multiple nucleopolyhedrovirus Hub1 and its field efficacy against S.frugiperda

草地贪夜蛾Spodoptera frugiperda是一种破坏性极强的害虫,严重威胁粮食安全。草地贪夜蛾核型多角体病毒Hub1 (S. frugiperda nuclear polyhedrosis virus Hub1,SpfrNPV Hub1)是首个专一性针对草地贪夜蛾的病毒杀虫剂,具有较好的应用前景。本研究采用药膜法评价不同温度、pH值、光照条件等处理对SpfrNPV Hub1活性及稳定性的影响,并开展了20亿病毒包涵体(polyhedral inclusion body,PIB)/mL SpfrNPV Hub1悬浮剂防治草地贪夜蛾田间药效试验。稳定性测定结果表明:SpfrNPV Hub1在pH 4和pH 7,4℃及25℃条件下稳定,在pH9条件下较不稳定,在54℃高温及4000 lx强光照射条件下不稳定。田间药效试验结果显示:20亿PIB/mL SpfrNPV Hub1悬浮剂对草地贪夜蛾有较好的防治效果,制剂用量为1500~1875mL/hm^(2)时,药后7 d和14 d的防效分别达81.18%~82.57%和81.23%~83.06%,持效期长。本研究明确了不同温度、pH值、光照条件对SpfrNPV Hub1活性及稳定性的影响,为指导应用SpfrNPV Hub1防治草地贪夜蛾提供了重要技术支撑。

Spodoptera frugiperda is a highly destructive pest that can damage crops and pose a serious threat to food security.The S.frugiperda multiple nucleopolyhedrovirus Hub1(SpfrNPV Hub1)represents the pioneering virus specifically targeting S.frugiperda,thereby exhibiting promising prospects for practical applications.This paper aims to assess the impact of various temperature,pH value,and light conditions on the stability of SpfrNPV activity using the residual film method.The stability test results demonstrated that SpfrNPV Hub1 exhibited stability at pH 4 and pH 7,as well as at temperatures of 4℃and 25℃.However,it displayed reduced stability at pH 9 and proved to be unstable under conditions of 54℃and 4000 lx illumination.The results of field trials revealed that SpfrNPV Hub12 billion PIB(polyhedral inclusion body)/mL SC effectively controlled S.frugiperda.When applied at a dose of 1500-1875 mL/hm^(2),the control efficiency remained consistently high,ranging from 81.18%to 82.57%at 7 days after application and from 81.23%to 83.06%at 14 days after application,indicating long-lasting effect.This study provides valuable insights into the impact of temperature variations,pH levels,and light conditions on the activity of SpfrNPV Hub1 while offering essential technical guidance for its application in controlling S.frugiperda.