响应面优化超声辅助乙醇提取银杏叶莽草酸工艺

Response Surface Optimization of Ultrasound-assisted Ethanol Extraction of Shikimic Acid From Ginkgo biloba Leaves

以银杏干叶为试材,采用超声辅助70%乙醇提取莽草酸,通过单因素试验和响应面试验优化提取工艺,研究了料液比、提取温度、提取时间、提取次数对莽草酸提取得率的影响,优化了银杏叶莽草酸的最佳提取工艺,以期为银杏叶莽草酸的提取提供参考依据。结果表明:最佳工艺条件为料液比1∶8 g·mL^(-1),提取温度66℃,提取时间65 min(每提取1次),提取次数2次。该条件下测得银杏叶莽草酸提取得率量为1.805%,与理论得率(1.883%)接近。

Shikimic acid was extracted from dried leaves of Ginkgo biloba L.by ultrasonic-assisted 70%ethanol.The extraction process was optimized by single factor experiment and response surface experiment.The effects of material-liquid ratio,extraction temperature,extraction time and extraction times on the extraction rate of shikimic acid were studied,and the optimum extraction process was optimized,in order to provide reference for the extraction of shikimic acid from Ginkgo biloba leaves.The results showed that the optimum technological conditions were as follows,material-liquid ratio 1∶8 g·mL-1,extraction temperature 66℃,extraction time 65 minutes(once for each extraction)and extraction times twice.Under these conditions,the extraction yield of shikimic acid from Ginkgo biloba leaves was 1.805%,which was close to the theoretical yield(1.883%).