miR-30a-5p、SOCS-3在复发性口腔溃疡患儿血清中的表达及其对再复发的预测价值

Expression of miR-30a-5p and SOCS-3 in serum of children with recurrent oral ulcer and their predictive value for recurrence

目的探讨微小RNA(miR)-30a-5p与细胞因子信号转导抑制因子-3(SOCS-3)在复发性口腔溃疡(ROU)患儿血清中的表达水平及其对再复发的预测价值。方法选取2020年1月至2022年3月石家庄市第二医院收治的610例ROU患儿作为研究组,同时期在该院进行体检的610例健康儿童为对照组。采用酶联免疫吸附试验检测血清SOCS-3表达水平,采用实时荧光定量聚合酶链反应检测血清miR-30a-5p表达水平。研究组患儿痊愈后进行1年随访,根据其是否复发分为复发组与未复发组。采用多因素Logistic回归分析ROU患儿再复发的影响因素。绘制受试者工作特征(ROC)曲线分析血清SOCS-3与miR-30a-5p单独及二者联合检测对ROU患儿复发的预测价值。结果与对照组比较,研究组血清SOCS-3表达水平降低,miR-30a-5p表达水平升高,差异均有统计学意义(P<0.05)。研究组ROU患儿随访1年无病例脱落,复发组纳入120例患儿,未复发组纳入490例患儿。复发组有ROU家族史、饮食类型偏荤、牙刷刷毛偏硬、合并消化系统疾病比例及病程、血清TNF-α、IL-2水平均高于未复发组,每天刷牙次数≥2次、每天睡眠时间≥8 h比例及血清SOCS-3表达水平均低于未复发组,miR-30a-5p表达水平高于未复发组,差异均有统计学意义(P<0.05)。两组性别、年龄、每次刷牙时间、学习压力情况比较,差异均无统计学意义(P>0.05)。多因素Logistic回归分析结果显示,饮食类型偏荤、牙刷刷毛偏硬、合并消化系统疾病、血清TNF-α、IL-2水平升高、miR-30a-5p表达水平升高均是ROU患儿再复发的危险因素(P<0.05),每天刷牙次数≥2次、每天睡眠时间≥8 h、血清SOCS-3表达水平升高均为ROU患儿再复发的保护因素(P<0.05)。ROC曲线分析结果显示,血清SOCS-3、miR-30a-5p表达水平单独及联合预测ROU患儿再复发的曲线下面积(AUC)分别为0.827、0.805、0.900,二者联合预测的AUC高于二者单独预测的AUC(Z=2.067、2.520,P<0.05)。结论ROU患儿血清SOCS-3表达水平降低、miR-30a-5p表达水平升高,且再复发患儿血清SOCS-3表达水平更低,miR-30a-5p表达水平更高,二者均对ROU患儿再复发具有预测效能,且联合预测效果更好。

Objective To investigate the expression levels of microRNA(miR)-30a-5p and suppressor of cytokine signaling 3(SOCS-3)in serum of children with recurrent oral ulcer(ROU)and their predictive value for recurrence.Methods A total of 610 children with ROU admitted to the Second Hospital of Shijiazhuang from January 2020 to March 2022 were selected as the study group,and 610 healthy children who underwent physical examination in the hospital during the same period were selected as the control group.The expression level of SOCS-3 in serum was detected by enzyme-linked immunosorbent assay,and the expression level of miR-30a-5p in serum was detected by real-time fluorescent quantitative polymerase chain reaction.The children in the study group were followed up for 1 year after recovery,and were divided into recurrence group and non-recurrence group according to the presence or absence of recurrence.Multivariate Logistic regression was used to analyze the influencing factors of ROU recurrence in children.The receiver operating characteristic(ROC)curve was drawn to analyze the predictive value of serum SOCS-3 and miR-30a-5p alone or in combination for the recurrence of ROU in children.Results Compared with the control group,the expression level of serum SOCS-3 in the study group was decreased,and the expression level of miR-30a-5p was increased,and the differences were statistically significant(P<0.05).ROU children in the study group were followed up for 1 year,and no ROU cases dropped out.A total of 120 children were included in the recurrence group and 490 children were included in the non-recurrence group.The proportion of ROU family history,meat diet type,hard toothbrush,digestive system diseases and course of disease,serum TNF-αand IL-2 levels in the recurrence group were higher than those in the non-recurrence group.The proportion of brushing teeth≥2 times a day,sleeping time≥8 hours a day and serum expression of SOCS 3 level in the recurrence group were lower than those in the non-recurrence group.,the expression level of miR-30a-5p in the recurrence group was higher than that in the non-recurrence group,and the differences were statistically significant(P<0.05).There was no significant difference in gender,age,time of brushing teeth each time,and learning pressure between the two groups(P>0.05).The results of multivariate Logistic regression analysis showed that the type of diet with meat,hard toothbrush,complicated with digestive system diseases,increased serum TNF-αand IL-2 levels,and increased miR-30a-5p expression level were all risk factors for recurrence of ROU children(P<0.05).The frequency of brushing teeth≥2 times per day,sleep time≥8 hours per day,and increased serum SOCS-3 expression level were protective factors for recurrence of ROU children(P<0.05).The results of ROC curve analysis showed that the area under the curve(AUC)of serum SOCS-3 and miR-30a-5p expression levels alone and in combination to predict the recurrence of ROU children were 0.827,0.805,and 0.900,respectively.The AUC of combined prediction was higher than that of single prediction(Z=2.067,2.520,P<0.05).Conclusion The expression level of serum SOCS-3 in children with ROU is decreased,the expression level of miR-30a-5p is increased,and the expression level of serum SOCS-3 in children with recurrence is lower,and the expression level of miR-30a-5p is higher.Both of them have predictive efficacy for the recurrence of ROU children,and the combined prediction effect is better.