Effects of water extract from Cornus controversa on the expression levels of DiSOC1-b and DiCCoAOMT1 genes related with the growth of dove tree seedlings

灯台树水提物对珙桐幼苗生长相关基因DiSOC1-b和DiCCoAOMT1表达水平的影响

Although characterization of plant interactions has become a research hotspot to assess the adaptability of endangered plants,the underlying molecular basis remains elusive.Dove tree(Davidia involucrata)seedlings were watered with distilled water(CK),leaf water extract(0.025 g mL^(-1))and branch water extract(0.1 g mL^(-1))from Cornus controversa,respectively.Subsequently,the morphology,biomass and gene expression levels of DiSOC1-b and DiCCoAOMT1 were analyzed.The results showed that morphological traits and biomass accumulation of D.involucrata seedlings were decreased by the addition of leaf water extracts,and increased by branch water extracts.Moreover,the gene expression level of DiSOC1-b was signifcantly down-regulated,while the gene expression level of DiCCoAOMT1 was signifcantly up-regulated in the stems and roots of D.involucrata upon treatment with leaf water extracts of C.controversa.In contrast,the gene expression level of DiSOC1-b was signifcantly up-regulated in the leaves and stems,while the gene expression level of DiCCoAOMT1 was signifcantly down-regulated in the roots of D.involucrata upon treatment with branch water extracts of C.controversa.In addition,the expression level of DiSOC1-b was positively correlated with most of morphological traits and total biomass(P<0.05),while DiCCoAOMT1 was negatively correlated with the majority of morphological traits in D.involucrata seedlings(P<0.05).Taken together,these results suggest that water extracts from the leaves and branches of the C.controversa exhibit opposite allelopathic effects and affect the expression levels of genes related to growth(DiSOC1-b)and environmental adaptability(DiCCoAOMT1)in D.involucrata seedlings.

植物间的相互作用已成为评估濒危植物适应能力的研究热点,但其潜在的分子基础仍不清晰。本研究对比分析了浇灌灯台树(Cornus controversa)叶水提物(0.025 g mL^(-1))和枝水提物(0.1 g mL^(-1))后,珙桐(Davidia involucrata)幼苗的形态、生物量、DiSOC1-b和DiCCoAOMT1基因表达量的差异。结果表明:灯台树叶水提物显著抑制珙桐幼苗形态和生物量的积累,而枝水提物显著促进珙桐幼苗形态和生物量的积累。此外,灯台树叶水提物处理后DiSOC1-b基因表达显著下调,DiCCoAOMT1基因表达显著上调。而灯台树枝水提物处理后,DiSOC1-b基因在珙桐叶和茎中表达显著上调,DiCCoAOMT1基因在珙桐根中的表达显著下调。DiSOC1-b表达量与珙桐幼苗大部分形态性状和总生物量呈正相关(P<0.05),DiCCoAOMT1表达量与大部分形态性状呈负相关(P<0.05)。上述结果表明,灯台树叶、枝水提物具有相反的化感效应,并影响珙桐幼苗生长相关基因(DiSOC1-b)和环境适应性相关基因(DiCCoAOMT1)的表达水平。