芸薹属植物KNOX1基因家族的鉴定及生物信息学分析

Identification and Bioinformatics Analysis of KNOX1 Gene Family in Brassica

KNOX基因家族是编码同源异型盒蛋白的转录因子,在植物生长发育和形态建成过程中起着重要的调控作用,同源异型盒基因(Class 1 KNOTTED-like homeobox,KNOX1)作为其中的一个分类,在调节和保持茎尖分生组织细胞活力方面,特别是在叶的生长和表型的形成中起到了非常关键的作用。为了进一步研究KNOX1基因家族在芸薹属植物不同组织发育中的作用,本研究利用生物信息学分析对芸薹属部分植物全基因组KNOX1基因家族成员进行鉴定,并对其基因结构、基因共线性关系、保守结构域和系统进化关系等情况进行分析。结果表明,在芸薹属植物白菜、甘蓝、甘蓝型油菜、芥菜和黑芥中共获得37个KNOX1基因,均编码不稳定的亲水性蛋白,所有蛋白均包含KNOX1、KNOX2、ELK和Homeobox KN的特征性保守结构域。通过结合进化和结构分析,发现芸薹属植物KNOX1蛋白的种间同源性非常高,但KNOX1位点不完全符合三倍体进化学说。同时从基因表达模式分析发现,不同芸薹属植物的KNOX1基因在根、茎、叶、花和种子中表达量有所不同。本研究将为进一步揭示芸薹属植物中KNOX1基因的具体功能提供相关的理论依据。

The KNOX gene family is a transcription factor that encodes homeobox proteins and plays an important regulatory role in plant growth and morphogenesis.The homeobox genes(Class 1 KNOTTED-like homeobox,KNOX1),which one of the classifications,have the function in regulating and maintaining the activity of shoot apical meristem,especially for leaf development and phenotypic formation.In order to explore the role of KNOX1 in the development of different tissues in Brassica plants,this study used bioinformatics analysis to identify the whole genome KNOX1 family members of some Brassica plants,and analyzed their gene structure,gene expression,conserved domain and phylogenetic relationship.The results showed that a total of 37 KNOX1 genes were obtained in Brassica napus,Brassica rapa,Brassica oleracea,Brassica juncea and Brassica nigra,all of which encoded unstable hydrophilic proteins,and all members contained KNOX1,KNOX2,ELK and Homeobox KN characteristic conserved domains.According to evolutionary analysis and gene structure analysis,it was found that the interspecific homology of Brassica plant KNOX1 proteins was very high,while the KNOX1 site did not fully conform to the triploid evolution theory.Gene expression pattern analysis showed that the expression levels of KNOX1 genes in different Brassica plants were different in roots,stems,leaves,flowers and seeds.This study will provide a theoretical basis for further revealing the specific function of KNOX1 gene in Brassica plants.