蛋白激酶GmCIPK24正向调节大豆耐盐性

Protein Kinase GmCIPK24 Positively Regulates Salt Tolerance in Soybean

类钙调磷酸酶B蛋白(CBLs)及其互作蛋白激酶(CIPKs)构成的CBL-CIPK信号途径在植物非生物逆境胁迫响应中发挥重要作用,但其在豆类作物盐胁迫应答中的生物学功能尚不明确。本研究以大豆品种‘Williams 82’为试材,克隆到CIPK蛋白激酶编码基因GmCIPK24(Glyma.17G113700)。序列分析结果表明,GmCIPK24开放阅读框为1341 bp,编码427个氨基酸。进化树和蛋白结构分析结果表明GmCIPK24属于富含内含子亚族CIPK蛋白激酶,具有Ser/Thr激酶结构域和保守的NAF/FISL基序。Gm CIPK24启动子序列包含盐胁迫、ABA和干旱胁迫相关的顺式作用元件。进一步的RT-qPCR实验分析表明,GmCIPK24受盐、ABA、碱胁迫和渗透胁迫诱导表达。过表达GmCIPK24能够显著提高转基因大豆的耐盐性,表明GmCIPK24在提高大豆耐盐性方面发挥重要作用,为阐明大豆CBL-CIPK信号通路应答盐胁迫的功能和大豆耐盐育种提供了基础资料。

The CBL-CIPK signaling pathway is composed of calcineurin B-like proteins(CBLs)and CBL-interacting protein kinases(CIPKs),which play important roles in plant environmental stress responses.However,the biological functions of CBL-CIPK signaling pathway in legume crops salt stress response remain unclear.In this study,a CIPK protein encoding gene,GmCIPK24(Glyma.17G113700),was cloned from soybean cultivar'Williams 82'.Sequence analysis showed that the open reading frame of GmCIPK24 was 1341 bp,encoding 427 amino acids.Phylogenetic tree assay demonstrated that GmCIPK24 belonged to the intron-rich CIPK protein kinase group.Protein structure analysis illustrated that GmCIPK24 had a Ser/Thr kinase domain and a conserved NAF/FISL motif.The GmCIPK24 promoter sequence had cis-acting elements related to salt stress,ABA,and drought stress.Furthermore,RT-qPCR assays illustrated that the expression of GmCIPK24 was induced by salt stress,abscisic acid(ABA),alkaline stress,and osmotic stress.Overexpression of GmCIPK24 significantly improved the salt tolerance of transgenic soybean plants.These results suggest that GmCIPK24 plays an important role in improving soybean salt tolerance.Furthermore,this study provides basic information in elucidating the role of CBL-CIPK signaling pathway in soybean salt stress response and soybean salt tolerance breeding.