新疆褐牛ELOVL1、2、4、5、6和7基因的生物信息学及表达分析

Analysis on Bioinformatics and Expression of ELOVL1,2,4,5,6,and 7Genes in Xinjiang Brown Cattle

【目的】旨在探究ELOVL1、2、4、5、6和7基因在新疆褐牛各组织中的表达规律,为今后ELOVL家族基因功能的挖掘奠定基础。【方法】利用实时荧光定量PCR(Real-time PCR)技术,在mRNA水平上对ELOVL1、2、4、5、6和7基因在新疆褐牛不同组织中的表达水平进行检测,并利用生物信息学方法分析ELOVLs基因的结构、系统进化关系、表达情况以及ELOVLs蛋白的多序列比对。【结果】Motif分析结果显示,ELOVL基因家族有10个保守元件(Motif1—Motif10),长度处于20~41 AA之间。不同物种ELOVLs系统发育分析显示,ELOVL基因家族具有较高的保守性,其中牛与山羊、绵羊的聚类更为相近。mRNA表达量检测结果显示,ELOVL1、2、4、5、6和7基因在新疆褐牛各个组织中广泛表达,其中ELOVL1在小肠中的mRNA表达量极显著高于其他组织;ELOVL2在肝脏中的mRNA表达量极显著高于其他组织;ELOVL4在脾脏中的mRNA表达量极显著高于其他组织;ELOVL5和ELOVL6在脂肪组织中呈现高表达水平;ELOVL7在肾脏中表达量极显著高于其他组织。【结论】总之,ELOVL1、2、4、5、6和7基因在新疆褐牛不同组织中广泛表达,其中ELOVL5、6和7基因在脂肪组织中表达量较高,推测新疆褐牛ELOVL5、6和7基因与脂肪酸代谢有关。

【Objective】In order to study the expression of ELOVL1,2,4,5,6,and 7 genes in various tissues of Xinjiang brown cattle and to lay a foundation for further mining the functions of ELOVLs.【Methods】The mRNA expression levels of ELOVL1,2,4,5,6,and 7 genes in different tissues of Xinjiang brown cattle was detected by real-time PCR,and the gene structure,phylogenetic relationships,expression status,and multiple sequence alignment of ELOVLs proteins were analyzed by bioinformatics method.【Results】The Motif analysis results showed that there were 10 conserved elements(Motif1—Motif10) in ELOVLs genes,with lengths ranging from 20 to 41 AA.Phylogenetic analysis of ELOVLs in different species showed that the gene family was highly conserved,with Bos taurus,Capra hircus,and Ovis aries clustering more closely.The detection results of mRNA expression showed that ELOVL1,2,4,5,6,and 7 genes were widely expressed in various tissues of Xinjiang brown cattle.Specifically,the mRNA expression level of ELOVL1 in the small intestine was extremely significantly higher than that in other tissues,so was the expression of ELOVL2 in the liver,ELOVL4 in the spleen and ELOVL7 in the kidney,and ELOVL5 and ELOVL6 exhibited high expression levels in adipose tissues.【Conclusion】In summary,ELOVL1,2,4,5,6,and 7 genes were widely expressed in different tissues of Xinjiang brown cattle,among which,ELOVL5,6,and 7 genes were highly expressed in adipose tissues,indication that ELOVL5,6,and 7 genes were related to fatty acid metabolism in Xinjiang brown cattle.