GC-MS法分析不同品牌藏香产品的化学成分

GC-MS analysis of chemical constituents of Tibetan incense products from different brands

为分析名优品牌藏香产品的化学成分及特征物质信息,以正己烷-丙酮混合溶液作为提取溶剂,对藏香样品进行超声提取,所得提取液经过滤后,再进样至气相色谱-质谱联用仪中分析并鉴定化学成分,通过峰面积归一化法获得各化合物相对百分含量.结果表明,从A品牌藏香、B品牌藏香和C品牌藏香样品中分别鉴定出61、62和34种化合物,其中包括16种共有成分:雪松醇、异土木香内酯、土木香内酯、去氢木香内酯、表木栓醇、丁香酚、β-香树脂醇、24-norursa-3,12-dien-11-one、24-norursa-3,12-diene、无羁萜、α-香树脂醇、熊果醛、24-noroleana-3,12-diene、日柏酮、桉油烯醇、正二十九烷,上述共有成分平均相对含量依次为4.36%、4.35%、3.68%、3.19%、2.63%、2.43%、1.75%、1.53%、1.45%、1.31%、1.26%、0.90%、0.81%、0.59%、0.40%、0.33%,其中雪松醇是藏香样品中平均含量最高的化合物.另外,这些共有成分中有14种为萜类,且在上述3种样品中萜类总量占共有成分比例分别为93.29%、93.93%、86.66%,表明萜类成分是藏香共有成分中数量最多、相对含量占比最大的一类化合物.综上,通过GC-MS法获得藏香产品化学成分及其特征成分,可为后续藏香产品的进一步研究和开发提供科学依据和参考.

In order to analyze the chemical composition and characteristic substance information of famous and excellent brand Tibetan incense products,the filtrate of Tibetan incense samples extracted by ultrasonic with n-hexane acetone mixture was analyzed by gas chromatography-mass spectrometry(GC-MS).The chemical components of the extract of Tibetan incense products were analyzed and identified.The relative percentage content of each compound was obtained by peak area normalization method.The results showed that 61,62 and 34 compounds were identified from the three samples of Tibetan incense sample A,Tibetan incense sample B,and Tibetan incense sample C,including 16 co-constituents:cedrol,isoalantolactone,alantolactone,dehydrocostus lactone,epifriedelinol,eugenol,β-amyrin,24-norursa-3,12-dien-11-one,24-norursa-3,12-diene,friedelan-3-one,α-amyrin,ursolic aldehyde,24-noroleana-3,12-diene,hinokione,(-)-spathulenol,nonacosane,The average relative contents of the above common components were 4.36%,4.35%,3.68%,3.19%,2.63%,2.43%,1.75%,1.53%,1.45%,1.31%,1.26%,0.90%,0.81%,0.59%,0.40%and 0.33%respectively.Cedrol was the compound with the highest average content in Tibetan incense samples.In addition,14 of these common components were terpenes,and the total terpenes in the above three samples accounted for 93.29%,93.93%and 86.66%of the common components,respectively,indicating that the terpenoids were the most numerous and the largest proportion of the relative content of the common components of Tibetan incense.In conclusion,the chemical components and characteristic components of Tibetan incense products obtained by GC-MS can provide scientific basis and reference for further research and development of Tibetan incense products.