离子色谱法同时测定贝叶经碎片中5种无机阴离子含量

Simultaneous determination of five inorganic anions in palm-leaf manuscript fragments by ion chromatography

建立离子色谱法检测贝叶经碎片中5种主要无机阴离子F^(-)、Cl^(-)、SO_(4)^(2-)、NO_(3)^(-)、H_(2)PO_(4)^(-)含量。淋洗液为30mmol/L KOH溶液,等浓度淋洗,流量为1.0 mL/min,5种阴离子的质量浓度在0~100 mg/L内与对应组分的色谱峰面积线性相关,相关系数均不小于0.9996,检出限为0.2482~0.4712 mg/L。对贝叶经样品进行重复性实验,5种离子的相对标准偏差1.49%~4.35%(n=7),加标回收率为96.4%~105.5%。该方法测定结果准确、可靠、操作简单、快速,适用于贝叶经碎片中主要无机离子含量的测定,对贝叶经病害的机理研究有着重要的意义。

An ion chromatography method was established for the determination of five major inorganic anions F^(-),Cl_(-),SO_(4)^(2-),NO_(3)^(-),and H_(2)PO_(4)^(-)in palm-leaf manuscript fragments.The elution solution was KOH solution at a concentration of 30 mmol/L,and the experiments were carried out at an isocratic elution rate of 1.0 mL/min.The mass concentration of the five anions was linearly related to the chromatographic peak area of the corresponding components in the range of 0-100 mg/L,the linear correlation coefficients were not less than 0.9996,and the detection limits were 0.2482-0.4712 mg/L.The reproducibilities of the palm-leaf manuscripts samples were investigated,and the relative standard deviations(RSDs)of the five ions were 1.49%-4.35%(n=7),and the recoveries were 96.4%-105.5%.The method is accurate,reliable,simple,rapid,and suitable for the determination of major inorganic ions in palm-leaf manuscripts,which is of great significance for the study of the mechanism of palm-leaf manuscripts diseases.