高效液相色谱法测定化妆品中补骨脂酚的不确定度评定

Uncertainty evaluation for determination of bakuchiol in cosmetics by high performance liquid chromatography

采用高效液相色谱法测定化妆品中补骨脂酚的含量,建立不确定度测量的数学模型,评定补骨脂酚含量的不确定度。通过对测量检测过程中不确定度的来源进行分析,量化各个测量不确定度分量,合成了相对标准不确定度和扩展不确定度。结果表明,当化妆品中补骨脂酚的质量分数为558.4μg/g时,扩展不确定度为18.76μg/g(k=2,P=95%)。测量不确定度的主要来源为标准溶液配制、样品溶液的配制和标准曲线拟合。在实验过程中可通过选用更为精准的量具,增加平行试验的次数来提高测量结果的准确性。

A high performance liquid chromatography method was used to determine the content of bakuchiol in cosmetics,and a mathematical model for the uncertainty measurement was established to evaluate the uncertainty of the content of bakuchiol.The sources of uncertainty in the measurement and testing process were analyzed,and each measurement uncertainty component was quantified to synthesize the relative standard uncertainty and extended uncertainty.The results indicated that when the content of bakuchiol in the sample was 558.4μg/g,the extended uncertainty was 18.76μg/g(k=2,P=95%).The uncertainty of this method mainly comes from the preparation of standard solution and sample solution,as well as the fitting of standard curve.The accuracy of the measurement results can be improved by choosing high precision glass gauge and increasing the number of parallel tests during the experimental process.