侧位液压冲击脑损伤诱发大鼠血脑屏障通透性改变

Lateral fluid percussion injury induces changes of blood-brain barrier permeability in rats

目的:探讨侧位液压损伤(LFPI)对大鼠血脑屏障完整性的影响及分子机制。方法:制备大鼠LFPI模型,利用神经功能缺损严重程度评分(NSS)评分评估大鼠的神经功能损害,通过干/湿比检测和伊文斯蓝渗透实验评估大鼠血脑屏障完整性,利用Western Blot检测脑组织中闭锁小带蛋白1(ZO-1)、闭合蛋白(occludin)和水通道蛋白4(AQP4)的表达。结果:LFPI导致大鼠神经功能发生障碍,脑含水量增加,伊文斯蓝通透增加,同时occludin和ZO-1表达下降,而AQP4表达升高。结论:LFPI能够导致大鼠脑水肿和血脑屏障完整性破坏。

Objective:To investigate the effect of lateral hydraulic injury(LFPI)on the integrity of blood-brain barrier in rats and its molecular mechanism.Methods:The rat LFPI model was prepared.Neurological impairment of rats was assessed by neurological severity score(NSS).The integrity of blood-brain barrier was assessed by dry/wet ratio test and Evans blue penetration test.The expressions of zonula occludens-1(ZO-1),occludin,and aquaporin 4(AQP4)were detected by Western Blot.Results:LFPI resulted in neurological dysfunction,increased cerebral water content,increased Evans blue permeability,decreased occludin and ZO-1 expression,and increased AQP4 expression.Conclusion:LFPI can cause cerebral edema and damage the integrity of blood-brain barrier in rats.