外源水杨酸干预高钙胁迫下苍术差异代谢物的分析

Analysis of Differential Metabolites of Atractylodes rhizoma under High Calcium Stress Intervention with Exogenous Salicylic Acid

利用代谢组学方法筛选了外源水杨酸干预高钙胁迫下苍术差异代谢物。结果显示,高钙胁迫组与对照组之间的显著差异代谢物有55个,其中26个上调,29个下调,且差异代谢物主要集中在脂质类。水杨酸干预高钙胁迫组与高钙胁迫组之间的显著差异代谢物有38个,其中26个上调,12个下调,且差异代谢物主要集中在脂质和氨基酸类。HCA分析表明,d-脱硫生物素、异锗脲、猴头菇素C、11-脱乙酰戊二酸11-(3-羟基-3-甲基丁酸)、9,10-二羟基-12Z-十八碳一烯酸、13(S)-氢过氧-(9Z,11E,15Z)-十八碳三烯酸、Nap-Asn-OH、肉豆蔻酰甘氨酸、玻璃蛋白C等代谢物含量在高钙胁迫下上升或下降,水杨酸干预后含量有恢复,表明外源水杨酸能有效缓解高钙胁迫对苍术代谢的影响。

In this study,metabolomics was further used to screen differential metabolites in Atractylodes rhizoma under high calcium(Ca)stress intervention with exogenous salicylic acid.Results showed that there were 55 metabolites showing significant differences between the high calcium stress group and control group,including 26 up-regulated and 29 down-regulated metabolites,mainly lipids.There were 38 metabolites showing significant differences between the the high calcium stress group interventing with salicylic acid and the high calcium stress group,including 26 up-regulated and 12 down-regulated metabolites,mainly lipids and carbohydrates.HCA analysis showed that the content of some metabolites,such as d-Desthiobiotin,Isogermafurene,Hericenone C,11-Deacetylvaltrate 11-(3-hydroxy-3-methylbutanoate),9,10-DHOME,13(S)-HPOT,Nap-Asn-OH,Myristoylglycine,Glabrin C,increased or decreased under high calcium stress and the content partially recovered after salicylic acid intervention,indicating that exogenous salicylic acid could effectively alleviate the effect of high calcium stress to the metabolism of A.rhizoma.