短梗霉普鲁兰多糖合成和调控的研究进展

Research progress in the synthesis and regulation of pullulan in Aureobasidium spp.

普鲁兰多糖是一种由短梗霉属真菌合成的胞外多糖。虽然普鲁兰多糖在生物技术的各个领域有着广泛的应用,但其生物合成与调控的具体机制有待进一步研究。近年来,研究人员利用分子生物学方法对普鲁兰多糖的合成与调控的分子机制进行阐明,发现以跨膜蛋白AmAgs2为关键多糖合成酶的合成途径,并揭示cAMP-Proteinkinase A(cAMP-PKA)、Target of rapamycin1(TORC1)、High osmotic glycerol 1(HOG1)和Sucrose nonfermentable 1(Snf1)信号通路参与普鲁兰多糖合成的调控。因此,本文对近年来该领域的研究进行综述,为真菌胞外多糖合成调控的机制提供研究参考,并为高产普鲁兰多糖细胞工厂的构建提供理论支持。

Pullulan is an exopolysaccharide produced by Aureobasidium spp.Despite its widespread biotechnological applications,the mechanisms underlying the biosynthesis and regulation of pullulan remain to be studied.In recent years,researchers have employed molecular biological techniques to elucidate the molecular mechanisms of pullulan synthesis and regulation.The transmembrane protein AmAgs2 is identified as a key enzyme for the synthesis of pullulan,and the cAMP-protein kinase A(cAMP-PKA),target of rapamycin 1(TORC1),high osmotic glycerol 1(HOG1),and sucrose nonfermentable 1(Snf1)signaling pathways are involved in the regulation of pullulan synthesis.We reviewed the research progress in this field,aiming to give insights into the research on the synthesis and regulation mechanisms of fungal extracellular polysaccharide and provide theoretical support for building cell factories with high yields of pullulan.