响应面法优化薏米红枣复合饮料配方及其抗运动疲劳活性研究

Response Surface Methodology Optimization of Coix and Jujube Compound Beverage Formula and the Anti-exercise Fatigue Activity

以薏米与红枣为原料,进行复合饮料的最佳工艺配方优化和抗运动疲劳活性研究。选取红枣汁添加量、薏米汁添加量、白砂糖添加量和柠檬酸添加量为影响因素,以薏米红枣复合饮料感官评分为响应值,采用响应面法优化薏米红枣复合饮料工艺配方,并对复合饮料进行抗运动疲劳活性研究。结果表明,饮料最优工艺配方为:红枣汁添加量26%,薏米汁添加量16%,白砂糖添加量8%,柠檬酸添加量0.15%,该配方制得的饮料综合感官评价较优,感官评分为96.12分;抗运动疲劳活性实验表明:与阴性对照组相比,连续灌胃薏米红枣复合饮料30 d的小鼠负重力竭游泳时间显著增加(P<0.05);该饮料有助于提高小鼠机体内肝糖原与肌糖原的储备水平;降低血液中乳酸与血尿素氮含量,有助于缓解小鼠机体疲劳,提高运动能力。综上,薏米红枣复合饮料对小鼠具有一定的抗疲劳作用。该研究结果可为饮料的开发提供新思路。

This study aimed to optimize the formulation and anti-fatigue activity of a compound beverage with coix rice and jujube as raw materials.The single factor experiments were conducted with the additions of red jujube juice,coix juice,white granulated sugar and citric acid as the experimental factors,then the response surface method was used to optimize the formulation of the compound beverage with sensory score as the response value,and the anti-fatigue activity was determined.The results showed that the optimal process formula of beverage was determined as follows:jujube juice addition of 26%,coix juice addition of 16%,white sugar addition of 8%,citric acid addition of 0.15%.The comprehensive sensory evaluation of the beverage was better with a sensory score of 96.12.The anti-fatigue activity test showed that the mice treated with coix and jujube compound beverage for 30 days exhibited a significantly increased exhaustive swimming time(P<0.05)compared with negative control group.Besides,the beverage could improve the storage levels of liver glycogen and muscle glycogen in mice,reduce the contents of blood lactic acid and blood urea nitrogen,which is helpful to relieve fatigue and improve exercise abilities of mice.In conclusion,coix and jujube compound beverage has certain anti-fatigue effects on mice,and the results can provide new ideas for the development of beverages and foods.