摘要
探究乳酸芽孢杆菌DU-106和植物乳杆菌混合菌发酵栀子对HepG2细胞酒精性氧化损伤的影响。采用CCK-8法探索最佳酒精性氧化损伤造模浓度和栀子提取液干预浓度,评价发酵栀子水提液对超氧化物歧化酶(SOD)、过氧化物酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活力、丙二醛(MDA)、谷胱甘肽(GSH)含量等抗氧化能力指标以及丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)等转氨酶类释放量、促炎细胞因子(IL-1β、IL-6、TNF-α)等肝损伤评价指标的影响。结果表明栀子提取液(Gardenia jasminoides Ellis extract,GE)和发酵栀子提取液(fermented Gardenia jasminoides Ellis extract,FGE)可显著提高酒精诱导HepG2细胞活力(P<0.05),与模型组(MN)相比,GE和FGE显著提高细胞的抗氧化应激能力,减轻脂质过氧化、减少GSH耗竭、降低细胞因子水平(P<0.01),减少细胞中AST、ALT和LDH的释放,改善酒精诱导的HepG2细胞膜的受损程度。其中,FGE具有显著的抗氧化和抗炎特性,在酒精性肝损伤中具有潜在的应用前景,可以保护HepG2细胞免受酒精性氧化损伤,为开发具有护肝功能的食品提供了有益的线索。
This study was to evaluate the effect of fermented Gardenia jasminoides Ellis by a mixture of Bacillus sp.DU-106 and Lactobacillus plantarum on alcoholic oxidative damage in HepG2 cells.The CCK-8 method was used to explore the optimal alcoholic oxidative damage modelling concentration and the intervention concentration of Gardenia jasminoides Ellis extract.The effect of fermented Gardenia jasminoides Ellis extract on antioxidant capacity indicators such as superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px)activity,malondialdehyde(MDA),glutathione(GSH)content,as well as the release of transaminase-like enzymes such as alanine aminotransferase(ALT),aspartate aminotransferase(AST),and lactate dehydrogenase(LDH),and indicators of evaluation of hepatic injury,such as pro-inflammatory cytokines(IL-1β,IL-6,and TNF-α),were detected.The results showed that Gardenia jasminoides Ellis extract(GE)and fermented Gardenia jasminoides Ellis extract(FGE)significantly increased the viability of ethanol-induced HepG2 cells(P<0.05),and compared with the model group(MN),GE and FGE significantly increased the cellular antioxidative stress,attenuated lipid peroxidation,reduced GSH depletion,lowered cytokine levels(P<0.01),reduced the release of AST,ALT and LDH in the cells,and improved ethanol-induced damage to HepG2 cell membranes.Among them,FEG had significant antioxidant and anti-inflammatory properties,which were potentially valuable for protecting HepG2 cells from alcoholic oxidative damage,and the present study provided useful clues for the development of food products with hepatoprotective function.
作者
廖静如
严静
赵文俊
陈冬球
王烈宇
张琦琳
杜冰
黎攀
LIAO Jingru;YAN Jing;ZHAO Wenjun;CHEN Dongqiu;WANG Lieyu;ZHANG Qilin;DU Bing;LI Pan(College of Food Science,South China Agriculture University,Guangzhou 510642,China)
出处
《食品工业科技》
CAS
北大核心
2024年第21期350-357,共8页
Science and Technology of Food Industry
基金
财政部和农业农村部:国家现代农业产业技术体系(CARS-21)
广东省重点领域研发计划(No:2020B020226008)。