城市污水处理厂病原菌多样性及健康风险评价

Evaluation of Pathogenic Bacterial Diversity and Health Risks in Urban WWTPs

污水中普遍存在大量病原菌,污水处理厂作为接收和处理污水的主要场所,在控制病原菌污染和保障再生水安全中发挥重要作用。研究利用16S r RNA测序技术和实时荧光定量PCR技术对中国北方一座污水处理厂夏、冬两季的细菌群落结构和病原细菌存在情况进行分析。研究结果表明,共检出27个病原菌菌属,其中假单胞菌属(Pseudomonas)、不动杆菌属(Acinetobacter)是主要病原菌属。经过一系列工艺处理,出水中含有病原菌属数目和含量明显降低,但发现常规出水检测指标大肠杆菌不能指示其他病原菌的存在情况和风险水平,提示仍需引入新的检测指标减少再生水回用的病原菌风险。

A large number of pathogenic bacteria are commonly found in wastewater,and wastewater treatment plants(WWTPs),as the main sites for receiving and treating wastewater,play an important role in controlling pathogenic bacterial contamination and ensuring the safety of reclaimed water.In this study,the bacterial community structure and the presence of pathogenic bacteria in a WWTP in northern China during summer and winter were analyzed using 16S rRNA sequencing and real-time fluorescence quantitative PCR techniques.The results showed that a total of 27 pathogen-containing bacterial genera were detected,among which Pseudomonas and Acinetobacter were the main pathogenic genera.After a series of process treatment,the number and content of pathogenic genera in the effluent were significantly reduced,but it was found that the conventional effluent testing index Escherichia coli could not indicate the presence and risk level of other pathogenic bacteria,suggesting that new testing indices still need to be introduced to reduce the risk of pathogenic bacteria in reclaimed water reuse.