清肺通络方对急性肺损伤后大鼠肺纤维化的影响

Effect of Qingfei Tongluo Prescription on Pulmonary Fibrosis Following Acute Lung Injury in Rats

目的:探索清肺通络方对内毒素诱导的急性重度肺损伤大鼠早期肺纤维化的抑制作用。方法:大鼠随机分为6组:空白组,模型组,地塞米松组、清肺通络方低、中、高剂量组(10,20,30g·kg^(-1))。苏木素-伊红染色观察肺组织病变,Masson染色分析肺纤维化程度。ELISA实验检测各组大鼠血浆中IL-1β和TNF-α的水平。结果:急性肺损伤大鼠血浆中TNF-α和IL-1β表达水平显示:模型组大鼠IL-1β和TNF-α水平高于空白组(P<0.05),清肺通络方各组(10,20,30g·kg^(-1))大鼠血浆中IL-1β和TNF-α水平均低于模型组(P<0.05),清肺通络方各组(10,20,30g·kg^(-1))大鼠血浆中IL-1β和TNF-α水平均低于地塞米松组(P>0.05),清肺通络方各组(10,20,30g·kg^(-1))之间无显著统计学差异(P>0.05)。苏木素-伊红染色检查显示:地塞米松组和清肺通络方各组肺组织病理损伤程度明显较模型组减轻,肺泡腔及肺泡隔结构尚存,肺泡间隔存在局部变厚,肺泡较少存在融合。Masson染色结果显示:模型组大鼠肺组织中可见大量被染成蓝色的胶原纤维沉积区域,其纤维化程度相较于地塞米松组和清肺通络方各组明显增加,地塞米松组大鼠肺组织仅有少量的区域被染成蓝色,纤维化程度最轻,地塞米松组和清肺通络方各组其纤维化程度与空白组相比无明显差异或差异较小。结论:清肺通络方可降低急性肺损伤后大鼠血浆炎性因子IL-1β和TNF-α水平,抑制急性肺损伤后早期肺纤维化程度。

Objective:To investigate the inhibitory effect of Qingfei Tongluo Prescription on early pulmonary fibrosis in rats with acute severe lung injury induced by endotoxin.Methods:Rats were randomly divided into six groups:blank group,model group,dexamethasone group,and Qingfei Tongluo Prescription low,medium,and high dose groups(10,20,30 g·kg-1).Pathological changes in lung tissue were observed using hematoxylin-eosin staining,and the degree of pulmonary fibrosis was analyzed by Masson staining.Plasma levels of IL-1βand TNF-αwere detected by ELISA.Results:Plasma levels of IL-1βand TNF-αin the model group were significantly higher than those in the blank group(P<0.05).In the Qingfei Tongluo Prescription groups(10,20,30 g·kg-1),IL-1βand TNF-αlevels were significantly lower than those in the model group(P<0.05).Although IL-1βand TNF-αlevels in the Qingfei Tongluo Prescription groups were lower than those in the dexamethasone group,the difference was not statistically significant(P>0.05).Hematoxylin-eosin staining showed that lung tissue damage in the dexamethasone group and Qingfei Tongluo Prescription groups was significantly reduced compared to the model group,with preserved alveolar cavities and septa,and less alveolar thickening and fusion.Masson staining revealed substantial collagen fiber deposition(stained blue)in the lung tissue of the model group,indicating increased fibrosis.In contrast,minimal blue staining and the least fibrosis were observed in the dexamethasone group,with similar findings in the Qingfei Tongluo Prescription groups.Conclusion:Qingfei Tongluo Prescription can reduce plasma levels of inflammatory factors IL-1βand TNF-αand inhibit early pulmonary fibrosis following acute lung injury in rats.