成年女性抑郁症患者血浆鞘磷脂组成特点与抑郁症状的相关性分析

Correlation analysis between the characteristics of plasma sphingomyelin composition and depressive symptoms in adult female patients with depression

目的分析成年女性抑郁症患者血浆鞘磷脂(SM)组成特点与抑郁症状的相关性。方法选取2020年5月至2021年3月我院收治的28例女性抑郁症患者作为观察组,同期招募25名健康女性作为对照组。采用汉密尔顿抑郁量表(HAMD)、汉密尔顿焦虑量表(HAMA)评估抑郁、焦虑严重程度,采用液相色谱-质谱联用技术(LC-MS)检测血浆中SM水平,分析SM组成特点与抑郁症状的相关性。结果观察组的HAMD、HAMA评分高于对照组(P<0.001)。观察组的SM总量水平显著低于对照组(P=0.018)。观察组的SM(t40∶1)+HCOO、SM(d40∶0)+H、SM(d36∶4)+H、SM(d30∶2)+H水平显著低于对照组(P<0.05)。Spearman相关性分析结果显示,SM差异分子与HAMA、HAMD评分呈负相关(P<0.05)。受试者工作特征(ROC)曲线结果显示,SM(d40∶0)+H、SM(t40∶1)+HCOO、SM(d36∶4)+H、SM(d30∶2)+H的曲线下面积(AUC)分别为0.903、0.807、0.803、0.727。结论抑郁症患者的血浆SM组成特点与抑郁症状呈负相关,SM(d40∶0)+H、SM(t40∶1)+HCOO、SM(d36∶4)+H、SM(d30∶2)+H分子可能是诊断女性抑郁症的潜在生物学标志物。

Objective To analyze the correlation between the characteristics of plasma sphingomyelin(SM)composition and depressive symptoms in adult female patients with depression.Methods A total of 28 female patients with depression admitted in our hospital from May 2020 to March 2021 were selected as observation group,and 25 healthy women were recruited as control group during the same period.The Hamilton Depression Scale(HAMD)and Hamilton Anxiety Scale(HAMA)were used to evaluate the severity of depression and anxiety,and the level of SM in plasma was detected by liquid chromatography-mass spectrometry(LC-MS),and the correlation between SM compositional characteristics and depressive symptoms were analyzed.Results The HAMD and HAMA scores of the observation group were higher than those of the control group(P<0.001).The total amount of SM level in the observation group was significantly lower than that in the control group(P=0.018).The levels of SM(t40∶1)+HCOO,SM(d40∶0)+H,SM(d36∶4)+H and SM(d30∶2)+H in the observation group were significantly lower than those in the control group(P<0.05).Spearman correlation analysis showed that SM different molecules were negatively correlated with HAMA and HAMD scores(P<0.05).The results of receiver operating characteristic(ROC)curve showed that the area under the curve(AUC)of SM(d40∶0)+H,SM(t40∶1)+HCOO,SM(d36∶4)+H and SM(d30∶2)+H were 0.903,0.807,0.803 and 0.727,respectively.Conclusion The characteristics of plasma SM composition in patients with depression were negatively correlated with depressive symptoms.SM(d40∶0)+H,SM(t40∶1)+HCOO,SM(d36∶4)+H and SM(d30∶2)+H molecules may be potential biomarkers for the diagnosis of female depression.