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津力达颗粒联合司美格鲁肽对肥胖非酒精性脂肪肝保护作用研究

Protective effect of Jinlida granule combined with semaglutide on non-alcoholic fatty liver disease
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摘要 目的探讨津力达颗粒联合司美格鲁肽对肥胖非酒精性脂肪肝(NAFLD)小鼠的保护作用。方法选取6周龄C57BL/6J雄性小鼠32只,随机取8只作为正常组(普通饲料喂养),其余小鼠给予高脂肪饲料喂养12周建立NAFLD模型。将造模成功的NAFLD小鼠随机分为NAFLD组、司美格鲁肽组、津力达联合司美格鲁肽组,每组8只。司美格鲁肽组给予司美格鲁肽30 nmol/kg腹腔内注射,津力达联合司美格鲁肽组在注射司美格鲁肽基础上给予津力达颗粒1.5 g/kg灌胃,正常组和NAFLD组给予生理盐水灌胃,均1次/d,连续干预12周。记录各组小鼠的体重,检测血脂指标[三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)]、肝功能指标[丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)]和空腹胰岛素(FINS)水平,HE染色、油红O染色、Masson染色和透射电镜观察肝脏组织病理学变化。结果NAFLD组小鼠体重及血清ALT、AST、TG、TC、LDL-C水平均明显高于正常组(P均<0.05);肝组织中可见大面积弥漫性肝细胞脂肪变性、肝细胞气球样变和淋巴细胞局灶性浸润,大量脂质沉积,线粒体肿胀,线粒体嵴断裂、减少,可见线粒体微自噬结构。与NAFLD组比较,司美格鲁肽组、津力达联合司美格鲁肽组小鼠体重及血清ALT、AST、TG、TC、LDL-C、FINS水平均明显降低(P均<0.05),肝组织病变明显减轻;与司美格鲁肽组比较,津力达联合司美格鲁肽组上述各指标更低(P均<0.05),肝组织病变更轻。结论津力达颗粒联合司美格鲁肽可明显调节NAFLD小鼠脂代谢,保护肝功能,减轻胰岛素抵抗,改善肝脏脂肪变性、脂质沉积、炎细胞浸润和线粒体微自噬结构。 Objective It is to explore the protective effect of Jinlida granule combined with semaglutide on obese mice with non-alcoholic fatty liver disease(NAFLD).Methods Thirty-two 6-week-old C57BL/6J male mice were selected,in which 8 mince were randomly selected as normal group fed with normal diet,and the other mice were fed with high-fat diet for 12 weeks to establish NAFLD models.The successfully modeled NAFLD mice were randomly divided into NAFLD group,semaglutide group,Jinlida combined with semiglutide group(combination group),with 8 mice in each group.The semaglutide group was given semiglutide 30 nmol/kg by intraperitoneal injection,the combination group was given Jinlida granules 1.5 g/kg by gavage on the basis of semaglutide by injection,the normal group and NAFLD group were given normal saline by gavage,all once daily,continuously treated for 12 weeks.The body weight of mice in each group was recorded,and the levels of blood lipids[triacylglycerol(TG),total cholesterol(TC),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C)],liver function indexes[alanine aminotransferase(ALT),aspartate aminotransferase(AST)]and fasting insulin(FINS)were detected.The histopathological changes of liver were observed by HE staining,oil red O staining,Masson staining and transmission electron microscopy.Results The body weight and serum levels of ALT,AST,TG,TC and LDL-C in the NAFLD group were significantly higher than those in the normal group(all P<0.05).Extensive diffuse hepatocyte steatosis,hepatocyte balloon-like degeneration,focal lymphocyte infiltration,large amount of lipid deposition,mitochondrial swelling,mitochondrial ridge breakage and reduction,and mitochondrial microautophagy were observed in the liver tissue.Compared with the NAFLD group,the body weight and serum levels of ALT,AST,TG,TC,LDL-C and FINS of mice in the semaglutide group and combination group were significantly decreased(all P<0.05),and the liver lesions were significantly alleviated.Compared with the semaglutide group,the decreases of the above indexes were more significant in the combination group(all P<0.05),and the liver lesions were more milder.Conclusion Jinlida granules combined with semaglutide can significantly regulate lipid metabolism,protect liver function,alleviate insulin resistance,improve liver steatosis,lipid deposition,inflammatory cell infiltration and mitochondrial microautophagy structure in NAFLD mice.
作者 牛姝 王晨曦 吴笛 孙政 陈杏 NIU Shu;WANG Chenxi;WU Di;SUN Zheng;CHEN Xing(Shijiazhuang People’s Hospital,Shijiazhuang 050011,Hebei,China;Hebei Medical University,Shijiazhuang 050000,Hebei,China;Shijiazhuang Hospital of Traditional Chinese Medicine,Shijiazhuang 050000,Hebei,China;Hebei General Hospital,Shijiazhuang 050000,Hebei,China)
出处 《现代中西医结合杂志》 CAS 2024年第16期2227-2232,共6页 Modern Journal of Integrated Traditional Chinese and Western Medicine
基金 河北省中医药管理局科研计划项目(2024127)。
关键词 非酒精性脂肪肝 肥胖 津力达颗粒 司美格鲁肽 线粒体 non-alcoholic fatty liver disease obesity Jinlida granules semaglutide mitochondrion
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