子痫前期患者的肠道菌群特征分析

Analysis of the characteristics of intestinal flora in patients with preeclampsia

目的分析子痫前期患者的肠道菌群特征。方法收集2020年7月至2022年7月在南昌大学第一附属医院接受治疗或住院的42例子痫前期患者(PE组)和20例正常孕妇(NP组)的粪便标本,通过16Sr RNA基因测序技术检测两组的肠道菌群,并分析肠道菌群特征。结果与NP组比较,PE组肠道微生物α多样性、β多样性降低(P<0.01)。在门水平上,两组粪便微生物主要由拟杆菌门、厚壁菌门、变形菌门、放线菌门和脱硫杆菌门等类别组成,其中拟杆菌门、厚壁菌门和变形菌门在肠道微生物群落中占据优势地位;在属水平上以拟杆菌属、普雷沃氏菌属、柔嫩梭菌属为优势菌属。结论PE患者较正常孕妇肠道菌群的多样性和丰富度降低,两者菌群结构存在差异。

Objective To analyze the characteristics of intestinal flora in patients with preeclampsia.Methods From July 2020 to July 2022,the stool samples of 42 patients with preeclampsia(PE group)and 20 normal pregnant women(NP group)who were treated or hospitalized in the First Affiliated Hospital of Nanchang University were collected.The intestinal flora of the two groups were detected by 16Sr RNA gene sequencing technology,and the characteristics of intestinal flora were analyzed.Results Compared with NP group,theαdiversity andβdiversity of intestinal microbes in PE group were decreased(P<0.01).At the phylum level,the fecal microbiota in both groups was mainly composed of Bacteroidetes,Firmicutes,Proteobacteria,Actinobacteria,and Desulfobacterota.Bacteroidetes,Firmicutes,and Proteobacteria were dominant in the intestinal microbial community.At the genus level,Bacteroides,Prevotella,and Faecalibacterium were the dominant genera.Conclusion The diversity and richness of intestinal flora in PE patients is lower than that in normal pregnant women,and there are differences in microflora structure between PE patients and normal pregnant women.