霞妃睡莲的组织培养及快繁技术研究

Study on tissue culture and rapid propagation technology of Nymphaea cv.Sunshine Princess

文章以霞妃睡莲(Nymphaea cv.Sunshine Princess)的根状茎为外植体,通过全面系统研究其初代培养、增殖、生根及移栽等不同阶段的培养条件,首次成功建立了睡莲的组培快繁技术体系。研究结果显示:固体培养基更适合霞妃睡莲的组织培养,且有利于提高睡莲的增殖率和人工操作效率;噻二唑苯基脲(TDZ)对睡莲不定芽的增殖诱导效果优于苄基腺嘌呤(BA),且两者一起使用具有协同效应,增殖率达到3.44且保持较好的生长状态;吲哚丁酸(IBA)对睡莲的生根诱导效果优于相同浓度的萘乙酸(NAA),活性炭的添加也有利于睡莲的生根诱导;适量的多菌灵能够提高睡莲组培苗的移栽成活率。该研究为睡莲种质资源的无菌保存及新品种的快速推广提供了有力的技术支持,对于加速睡莲种苗的规模化生产和推动国内外睡莲产业发展具有重要的现实意义。

In this paper,the rhizoid of Nymphaea cv.Sunshine Princess was used as explants,and the culture conditions at different stages of Nymphaea cv.Sunshine Princess such as primary culture,proliferation,rooting and transplanting were studied comprehensively and systematically,and the tissue culture and rapid propagation technology system of waterlily was successfully established for the first time.The results showed that the solid medium was more suitable for tissue culture of Nymphaea cv.Sunshine Princess,and was beneficial to increase the proliferation rate and manual operation efficiency of waterlily.The effect of TDZ on the proliferation of buds was better than that of BA,but the two had a synergistic effect,and the proliferation ratio reached 3.44 and the growth state was good.The rooting effect of IBA was better than that of NAA at the same concentration,and the addition of activated carbon was also beneficial to the rooting induction of waterlily.Proper dosage of carbendazim could improve the survival rate of waterlily tissue culture seedlings.This study provides strong technical support for the aseptic preservation of waterlily germplasm resources and the rapid promotion of new varieties,and has important practical significance for accelerating the large-scale production of waterlily seedlings and promoting the industrial development of waterlily at home and abroad.