桑树根系分泌物鉴定及其主要活性物质对桑树青枯菌生长的影响研究

Identification of mulberry root exudates and effects of main active compounds on the growth of Ralstonia pseudosolanacearum

为探讨桑树(Morus alba)根系分泌物对青枯病菌(Ralstonia pseudosolanacearum)的抑菌活性,本研究利用气相色谱-质谱联用(GC-MS)技术及平板抑菌试验研究了桑树根系分泌物主要活性物质对桑树青枯病菌(Ralstonia pseudosolanacearum)生长的影响。结果表明,桑树根系分泌物中含量最高的3种主要活性物质包括芥酸酰胺、油酸酰胺和溴樟脑,其中芥酸酰胺、油酸酰胺在根系分泌物中的质量占总质量的42.6%,溴樟脑占总质量的18.33%。三种主要的根系分泌物对桑树青枯菌MRS-5的生长均表现出明显的抑制作用,在1.5 g·L^(-1)时的抑菌效果最佳,抑菌率均达到90%以上,并能够显著抑制MRS-5生物膜的形成和运动能力。本研究揭示了桑树根系分泌物中主要活性物质的抑菌效果和潜在的防治机制,对理解和防控桑树青枯病提供科学和实践参考。

To investigate the antibacterial activity of mulberry(Morus alba)root exudates on the Ralstonia pseudosolanacearum,this study utilized gas chromatography-mass spectrometry technology and plate inhibition assays to examine the effect of the main active substances in mulberry root exudates on the growth of R.pseudosolanacearum.The results indicate that the predominant active substances in mulberry root exudates,including(Z)-13-docosenamide and(Z)-9-octadecenamide(42.6%),and(endo)-(−)-3-bromocamphor(18.33%),exhibited significant inhibitory effects on the growth of the mulberry bacterial wilt of MRS-5. The best inhibitory effect was observed at a concentration of 1.5 g·L^(-1), with inhibition rates exceeding90%. Moreover, these substances were able to significantly inhibit the biofilm formation and motility ofMRS-5. This study reveals the inhibitory effects and potential control mechanisms of the main active substancesin mulberry root exudates, contributing important scientific value and practical significance to theunderstanding and management of mulberry wilt disease.