姜黄素通过抑制微囊泡分泌影响肺腺癌细胞增殖和迁移的机制研究

Effect of Curcumin on Proliferation and Migration of Lung Adenocarcinoma Cells by Inhibiting the Secretion of Microvesicles

目的:探讨姜黄素对肺腺癌A549细胞系增殖、迁移的影响及其可能机制。方法:利用不同浓度姜黄素处理A549细胞系,提取上清微囊泡,流式鉴定并计数,进一步用姜黄素及姜黄素+微囊泡处理A549细胞,以细胞计数试剂(CCK8)检测细胞增殖活性,划痕实验、Transwell实验检测细胞迁移功能。结果:流式细胞仪分析结果显示,随着姜黄素浓度的增加,A549细胞分泌的微囊泡数量明显减少;在A549细胞系中加入姜黄素或姜黄素+微囊泡处理后,CCK8细胞增殖实验结果显示,姜黄素能抑制A549细胞增殖,微囊泡可逆转姜黄素抑制效应,划痕实验和Transwell实验结果显示,姜黄素能抑制A549细胞迁移,微囊泡可逆转姜黄素抑制作用,组间差异具有统计学意义(P<0.05)。结论:姜黄素能够减少A549细胞系微囊泡分泌,进而抑制肺腺癌增殖、迁移功能。

Objective To investigate the effect of curcumin on the proliferation and migration of lung adenocarcinoma A549 cell line and its possible mechanism.Methods A549 cell lines were treated with different concentrations of curcumin,and supernatant microvesicles were extracted,identified and counted by flow cytometry.A549 cells were further treated with curcumin and curcumin+microvesicles,and cell proliferation activity was detected by CCK8 assay.Cell migration function was detected by scratch assay and Transwell assay.Results Flow cytometry analysis showed that as the concentration of curcumin increased,the number of microvesicles secreted by A549 cells significantly decreased;After adding curcumin or curcumin+microvesicles treatment to A549 cell line,the results of CCK8 cell proliferation experiment showed that curcumin could inhibit A549 cell proliferation,microvesicles could reverse the inhibitory effect of curcumin,scratch test and Transwell test results showed that curcumin could inhibit A549 cell migration,microvesicles could reverse the inhibitory effect of curcumin,and the difference between groups was statistically significant(P<0.05).Conclusion Curcumin can reduce the secretion of microvesicles in A549 cell line,thereby inhibiting the proliferation and migration function of lung adenocarcinoma.