hsa_circ_0082002在原发性肝细胞癌患者血清中的表达及意义

Expression and significance of hsa_circ_0082002 in serum of patients with primary hepatocellular carcinoma

目的检测原发性肝细胞癌(HCC)患者血清中hsa_circ_0082002的表达水平,探讨其辅助诊断价值。方法选取2020年1月至2022年12月在该院就诊的HCC患者70例为HCC组,肝硬化(LC)患者55例为LC组,慢性乙型肝炎(CHB)患者55例为CHB组,体检健康者50例为对照组。采用实时荧光定量PCR检测各组血清hsa_circ_0082002相对表达水平,电化学发光法检测血清甲胎蛋白(AFP)水平,采用受试者工作特征(ROC)曲线评估hsa_circ_0082002和AFP对HCC的鉴别诊断效能。结果HCC组、LC组、CHB组和对照组血清hsa_circ_0082002相对表达水平分别为2.750(1.925,4.393)、2.199(1.416,2.850)、1.591(0.925,2.107)和1.035(0.666,1.680),HCC组血清hsa_circ_0082002相对表达水平高于LC组、CHB组和HC组,差异均有统计学意义(均P<0.05)。ROC曲线结果显示,hsa_circ_0082002、AFP鉴别诊断HCC的曲线下面积(AUC)分别为0.813、0.779,二者联合检测的AUC达0.871,其灵敏度为75.71%,特异度为83.13%。结论血清hsa_circ_0082002可能是HCC辅助诊断的一个生物学指标。

Objective To detect the expression level of hsa_circ_0082002 in serum of patients with primary hepatocellular carcinoma(HCC)and explore its auxiliary diagnostic value.Methods A total of 70 HCC patients treated in this hospital from January 2020 to December 2022 were selected as the HCC group,55 patients with liver cirrhosis(LC)were selected as the LC group,55 patients with chronic hepatitis B(CHB)were selected as the CHB group,and 50 healthy people were selected as the control group.The relative expression level of hsa_circ_0082002 in serum of each group was detected by real-time fluorescent quantitative PCR,and the level of serum alpha-fetoprotein(AFP)was detected by electrochemiluminescence.The receiver operating characteristic(ROC)curve was used to evaluate the differential diagnostic efficacy of hsa_circ_0082002 and AFP for HCC.Results The relative expression levels of serum hsa_circ_0082002 in HCC group,LC group,CHB group and control group were 2.750(1.925,4.393),2.199(1.416,2.850),1.591(0.925,2.107)and 1.035(0.666,1.680),respectively.The relative expression level of serum hsa_circ_0082002 in the HCC grou p was higher than those in the LC group,CHB group and HC group,and the differences were statistically significant(all P<0.05).The results of ROC curve showed that the area under the curve(AUC)of hsa_circ_0082002 and AFP in the differential diagnosis of HCC were 0.813 and 0.779,respectively.The AUC of combined detection of hsa_circ_0082002 and AFP was 0.871,with a sensitivity of 75.71%and specificity of 83.13%.Conclusion Serum hsa_circ_0082002 may be a biomarker for the diag nosis of HCC.