HBV相关肝细胞癌患者PPP2R3A基因的表达与预后相关性分析

Analysis of the correlation between the expression of PPP2R3A gene and prognosis in patients with HBV-related hepatocellular carcinoma

目的探讨蛋白磷酸酶2A调节亚基B″α(PPP2R3A)基因在HBV相关肝细胞癌患者中的表达水平以及与预后的关系。方法利用免疫组织化学方法检测PPP2R3A蛋白在82例乙肝相关性肝癌和21例良性肝病组织中的表达,采用RT-PCR方法检测mRNA水平。结果PPP2R3A在肝癌组织中的表达水平(95.12%)明显高于癌旁组织(43.9%)(P<0.05)。PPP2R3A蛋白的表达强度与肝癌患者的AFP值相关,具有统计学差异(P<0.05)。RT-PCR检测结果显示,肝癌组织中PPP2R3A mRNA中位表达水平为0.183(0.008~0.667),明显高于癌旁组织的0.098(0.004~0.583)(Z=6.839,P<0.05)。通过分析随访肝癌患者的生存期资料,结果发现PPP2R3A高表达组患者的总生存期(OS)的中位生存期为46.6个月,与低表达组的82.9个月比较差异有统计学意义(P<0.05)。PPP2R3A高表达组患者的RFS的中位生存期为18.3个月,低于低表达组的42.6个月(P<0.05)。PPP2R3A高表达组患者的PFS的中位生存期为15.9个月,低于低表达组的34.4个月(P<0.05)。PPP2R3A高表达组患者的DSS的中位生存期为70.5个月,低于低表达组的84.7个月(P<0.05)。结论PPP2R3A蛋白在HBV相关肝细胞癌组织及血清中呈高表达,且与肝癌的临床病理特征有相关性,PPP2R3A的高表达可能与肝癌的不良预后有关。

Objective To investigate the expression level of protein phosphatase 2A regulatory subunit B alpha(PPP2R3A)gene in patients with Hepatitis B virus(HBV)related hepatocellular carcinoma and its relationship with prognosis.Methods Utilizing the immunohistochemical method,we analyzed the expression levels of PPP2R3A protein in 82 samples of HBV-related hepatocellular carcinoma and 21 cases of benign liver disease.Additionally,the RT-PCR technique was applied to quantify the mRNA levels.Results Our findings revealed that the PPP2R3A protein expression in liver cancer tissue(95.12%)was substantially elevated compared to its levels in pericancerous tissue(43.9%),with statistical significance(P<0.05).There was a notable correlation between the expression intensity of PPP2R3A protein and the AFP value of liver cancer patients,exhibiting statistical difference(P<0.05).RT-PCR analysis indicated that the median mRNA expression level of PPP2R3A in HCC tissues was 0.183(0.008~0.667),which was significantly higher than that in adjacent tissues(0.098(0.004~0.583))(Z=6.839,P<0.05).By analyzing the survival data of follow-up HCC patients,it was found that the median overall survival(OS)of patients in the PPP2R3A high expression group was 46.6 months,which was significantly different from that in the low expression group(82.9 months)(P<0.05).The median survival of RFS in patients PPP2R3A the high-expression group was 18.3 months,which was lower than that in the low-expression group(42.6 months)(P<0.05).The median survival of PFS in patients PPP2R3A the high-expression group was 15.9 months,which was lower than that in the low-expression group(34.4 months)(P<0.05).The median survival of DSS in patients PPP2R3A the high-expression group was 70.5 months,which was lower than that in the low-expression group(84.7 months)(P<0.05).Conclusion The high expression of PPP2R3A protein in HBV-related hepatocellular carcinomar tissue and serum is related to the clinicopathological characteristics of liver cancer,which may be related to the poor prognosis of liver cancer.