山茶油对乳液中活性物质体外透皮吸收的影响

Effect of Camellia Japonica Oil on in vitro Transdermal Absorption of Active Substances in Emulsions

为了筛选合适的辅助促渗剂来提高山茶油的促渗能力,以离体猪皮肤为模型,通过体外改良Franz扩散池法,利用高效液相色谱法和气相色谱法定量分析茶多酚和1,3-丁二醇的经皮累积量和皮内滞留量,探究山茶油对乳液中活性物质体外透皮吸收的影响。结果显示,在乳液中质量分数3%山茶油具有最优促渗能力,质量分数0.5%的乙氧基二甘醇可提高山茶油对活性物质的促渗作用,而质量分数≥0.5%的月桂氮卓酮则抑制山茶油促渗。与山茶油促渗机制不同的乙氧基二甘醇可作为辅助促渗剂提高山茶油的促渗效果。

In order to screen suitable auxiliary pro-osmotic agents to improve the pro-osmotic ability of Camellia japonica oil,effects of Camellia japonica oil on the in vitro transdermal absorption of the active substances in emulsions were investigated by using in vitro modified Franz diffusion cell method.The isolated porcine skin as a model.Then high performance liquid chromatography(HPLC)and gas chromatography(GC)were used to quantitatively analyze the transdermal accumulations and intradermal retentions of tea polyphenols and 1,3-butanediol.The results showed that 3%(mass fraction)of Camellia japonica oil in the emulsion had the optimal permeation promoting ability,0.5%(mass fraction)of ethoxydiglycol improved the permeation promoting effect of Camellia japonica oil on the actives,and the addition of a certain concentration(≥0.5%,mass fraction)of lauric azadirachtinone inhibited the permeation promoting effect of Camellia japonica oil.Ethoxydiglycol,which had a different osmotic mechanism with Camellia japonica oil,could be used as an auxiliary osmotic agent to improve the osmotic effect of Camellia japonica oil.