芒果汁液体培养平菇菌丝体的优化

Optimization for Mycelia Cultivation of Pleurotus ostreatus with Mangifera indica Juice

以芒果汁添加比例、蛋白胨添加比例和菌丝接种量作为变量,研究优化平菇芒果汁液体培养基的营养配方。培养5 d平菇菌丝,通过测定菌丝干质量和以硫酸-苯酚法测定胞外多糖含量进行单一变量试验。同时,以L9(33)正交试验,最终确定最佳的液体培养基配方。结果表明,菌丝干质量随芒果汁添加量增加而先增后减,菌丝干质量在芒果汁添加量40%时最高;菌丝干质量和胞外多糖含量随蛋白胨添加先增后减,在蛋白胨添加比例为0.6%时最高;菌丝干质量和胞外多糖含量随接种量增加先增后减,在接种量为0.8%时最高。由正交实验可知,最佳的平菇芒果汁液体培养基营养配方的芒果汁添加量为40%、蛋白胨添加量为0.6%和接种量为0.8%。

In this experiment,the nutrient formula of Mangifera indica juice medium for Pleurotus ostreatus was optimized by taking the proportion of M.indica juice,the proportion of peptone and the amount of mycelium inoculation as the variables.The mycelia were cultured for 5 days,and the single variable experiment was carried out by measuring the dry weight of mycelia and the exopolysaccharide content by sulfuric acid-phenol method.At the same time,L9(33)orthogonal test was used to determine the optimal formula of liquid medium.The results showed that the dry weight of mycelia increased first and then decreased with the increases of M.indica juice proportion,and the dry weight of mycelia was the highest when the proportion of M.indica juice was 40%.The mycelial dry weight and extracellular polysaccharide content increased first and then decreased with peptone supplementation,and the highest value was reached when peptone supplementation was 0.6%.The mycelial dry weight and exopolysaccharide content increased first and then decreased with the increase of inoculation amount,and the highest value was reached when the inoculation amount was 0.8%.The orthogonal experiment showed that the optimal nutrient formula of P.ostreatus and M.indica juice medium was 40%M.indica juice,0.6%peptone and 0.8%inoculum.