杜鹃AQP基因家族的鉴定与生物信息学分析

IDENTIFICATION AND BIOINFORMATICS ANALYSIS OF AQP GENE FAMILY IN RHODODENDRON SIMSII

为了研究杜鹃水通道蛋白基因家族的生物学功能,本研究利用生物信息学方法在杜鹃基因组中鉴定了35个水通道蛋白基因。基因位置分析水通道蛋白基因在杜鹃13条染色体上不均匀分布。聚类分析显示杜鹃水通道蛋白可以分为5个亚家族(PIP、TIP、NIP、SIP和XIP)。启动子响应元件分析发现大部分AQP基因启动子区所含元件与激素调节、光响应和抗逆胁迫等密切相关。共线性分析表明有4对AQP基因出现了片段重复;Ka/Ks(同义替换/非同义替换)结果显示均小于1,表明这些基因经历了较强的纯化选择。本研究为了解杜鹃水通道蛋白家族的功能提供了理论基础。

To investigate the biological function of aquaporin gene family in Rhododendron simsii,a total of 35 AQP genes were identified in genome by bioinformatics methods.Gene location results showed that these genes were unevenly distributed on chromosomes.Phylogenetic analysis revealed that the AQP gene family could be divided into 5 subfamilies(PIP,TIP,NIP,SIP and XIP).Promoter response elements analysis demonstrated that the majority of the elements in the gene promoter region were closely associated with hormone regulation,light response,and stress resistance.Collinearity analysis showed that 4 pairs of fragments were discovered in 8 R.simsii AQP genes,with less than 1 value of Ka/Ks(nonsynonymous/synonymous),indicating that these genes had undergone strong purification selection during the evolution process.This study provides a theoretical basis for understanding the function of the aquaporin family in R.simsii.