高效液相色谱法测定奶油蛋糕中11种合成色素的含量

Determination of 11 Synthetic Pigments in Cream Cake by High Performance Liquid Chromatography

本文建立高效液相色谱法测定奶油蛋糕中11种人工合成色素含量的分析方法。采用正己烷、乙醇氨水溶液提取样品和ProElut PWA-2l固相萃取柱净化样品;以Waters C18色谱柱(250 mm×4.6 mm,5μm)为分离柱,以甲醇、乙酸铵为流动相进行梯度洗脱,外标法定量。结果表明,优化后的前处理方法的加标回收率明显提升;11种合成色素在0.2~10.0μg·mL^(-1)线性关系良好,相关系数均大于0.999;方法检出限和定量限符合国家标准规定,相对标准偏差为1.2%~2.7%,加标回收率为85.6%~93.6%。该方法前处理简便快速、准确灵敏、分离效果好,适用于在大批量奶油蛋糕中对11种人工合成色素进行同时检测。

This article establishes an analytical method for determining the content of 11 artificially synthesized pigments in cream cakes using high-performance liquid chromatography.Samples were extracted using n-hexane,ethanol ammonia solution,and purified using a ProElut PWA-2l solid-phase extraction column;using a Waters C18 column(250 mm×4.6 mm,5μm)as the separation column,gradient elution was performed with methanol and ammonium acetate as the mobile phases,and quantification was performed by external standard method.The results showed that the optimized pretreatment method significantly improved the spiked recovery rate;the linear relationship of 11 synthetic pigments is good between 0.2μg·mL^(-1) and 10.0μg·mL^(-1),with correlation coefficients greater than 0.999;the detection limit and quantification limit of the method comply with national standards,with a relative standard deviation of 1.2%to 2.7%and a spiked recovery rate of 85.6%to 93.6%.This method is simple,fast,accurate,sensitive,and has good separation effect in pre-processing,and is suitable for simultaneous detection of 11 artificial synthetic pigments in large quantities of cream cakes.