利用污泥厌氧化生产的甲烷作为碳源实现垃圾渗滤液脱氮的厌氧菌筛选研究

Optimization of Anaerobic Bacteria for Nitrogen Removal from Landfill Leachate Using Anaerobic Sludge to Produce Methane as A Carbon Source

垃圾渗滤液作为一种典型的含高氨氮高有机物的污水,其总氮浓度排放要求严格,而传统的异养反硝化需要额外的有机碳,并且会导致二次污染及更多CO_(2)的排放。反硝化厌氧甲烷氧化(DAMO)是一种利用甲烷(CH_(4))作为电子供体和碳源进行反硝化的过程,而厌氧消化可以利用污泥中的有机物作为发酵底物,在厌氧条件下生产CH_(4),同时将DAMO与厌氧氨氧化(Anammox)相结合实现垃圾渗滤液的深度和完全脱氮,是一种经济的解决方案。基于此背景,本论文筛选了市面上几种厌氧菌用于污泥产甲烷,从而为后续的深度脱氮实验提供合适的厌氧菌。通过对比后,筛选碧莱清高级厌氧菌CJW388品类作为市售高级厌氧菌剂代表,并以该厌氧菌为添加剂,研究了不同底物种类、底物浓度以及接种比例的条件影响。实验结果发现,在高浓度COD(5000 mg/L)情况下,添加市售菌剂对于乙酸钠和丙酸钠基质产甲烷的提升量和比例基本一致;在低浓度COD(1500 mg/L)进料情况下,添加市售菌剂对于乙酸钠基质产甲烷的提升量和比例远高于丙酸钠基质。

As a typical kind of wastewater containing high ammonia nitrogen and organic matter,it has strict requirements for total nitrogen concentration emissions of landfill leachate from garbage.However,traditional heterotrophic denitrification requires additional organic carbon and this method can lead to secondary pollution and much more CO_(2)emissions.Denitrifying anaerobic methane oxidation(DAMO)is a process that utilizes methane(CH_(4))as an electron donor and carbon source for denitrification.Moreover,anaerobic digestion can use organic matter in sludge as a fermentation substrate to produce CH_(4) under anaerobic conditions.At the same time,combining DAMO with anaerobic ammonia oxidation(Anammox)to achieve deep and complete denitrification of landfill leachate is an economical solution.Based on this background,this work focuses on the selection of several anaerobic bacteria on the market for sludge methane production,in order to provide suitable anaerobic bacteria for subsequent deep denitrification experiments.After comparison,the CJW388 class of advanced anaerobic bacteria from Biliqing is selected as a representative of commercially anaerobic bacterial agents.The anaerobic bacteria are used as an additive to investigate the effects of different substrate types,substrate concentrations,and inoculation ratios.The experimental results show that under high concentrations of COD(5000 mg/L),the addition of commercially microbial agents can result in a similar increase and proportion in methane production from sodium acetate and sodium propionate substrates;In the case of low concentration COD(1500 mg/L)feed,the addition of microbial agents can significantly increase the amount and proportion of methane production in sodium acetate substrate compared to sodium propionate substrate.