HPLC测定化妆品中粉防己碱的含量

Determination of Tetrandrine in Cosmetics by HPLC

建立化妆品中粉防己碱的含量测定方法,并测定不同剂型产品中含有粉防己碱的含量。采用高效液相色谱法测定化妆品中粉防己碱的含量,色谱柱:phenomenex Luna®C18(2)(250 mm×4.6 mm,5μm);流动相为甲醇∶0.2%三乙胺=75∶25;流速:1.0 mL/min;柱温:30℃;检测波长:280 nm。结果表明,粉防己碱在0.024μg~0.486μg范围内呈良好线性关系(R^(2)=0.9996),水剂样品平均回收率为98.96%,RSD=1.37%(n=9),膏霜样品的平均回收率为101.03%,RSD=1.87%(n=9),乳液样品的平均回收率为101.35%,RSD=1.91%(n=9)。该法简便、准确可行,可用于化妆品中粉防己碱的含量测定及质量控制。

To establish a HPLC method for determination of tetrandrine in cosmetics and determine the content of tetrandrine in different products.Analyses were performed on phenomenex Luna®C18(2)(250 mm×4.6 mm,5μm)column with methanol and 0.2%triethylamine water solution,which was used as mobile phases,with a flow rate of 1.0 mL/min.The detection wave length was 280 nm,column temperature was 30℃.Results showed that the linear relationship was good in the range of 0.024μg~0.486μg(R^(2)=0.9996).The average recovery of liquid samples was 98.96%and the RSD was 1.37%(n=9).The average recovery of cream samples was 101.03%and the RSD was 1.87%(n=9).The average recovery of emulsion samples was 101.35%and the RSD was 1.91%(n=9).The method is simple and accurate,and can be used for the determination and quality control of tetrandrine in cosmetics.